Combined effect of PLGA and curcumin on wound healing activity

• Published in: Journal of controlled release 2013-10, Vol.171 (2), p.208-215
• Main Authors: Chereddy, Kiran Kumar, Coco, Régis, Memvanga, Patrick B., Ucakar, Bernard, des Rieux, Anne, Vandermeulen, Gaëlle, Préat, Véronique
• Format: Article
• Language: English
• Subjects: additive effect; Angiogenesis; animal models; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - chemistry; Cell Line, Tumor; Cell Survival - drug effects; Collagen - metabolism; Curcumin; Curcumin - administration & dosage; Curcumin - chemistry; Drug delivery; encapsulation; Female; Glutathione Peroxidase - genetics; granulation tissue; histology; Keratinocytes - drug effects; Keratinocytes - metabolism; L-Lactate Dehydrogenase - metabolism; lactic acid; Lactic Acid - administration & dosage; Lactic Acid - chemistry; Mice; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; NF-kappa B - genetics; Peroxidase - metabolism; Poly (lactic-co-glycolic acid); Polyglycolic Acid - administration & dosage; Polyglycolic Acid - chemistry; polymers; reverse transcriptase polymerase chain reaction; Skin - injuries; Skin - metabolism; Skin wound healing; Tetrazolium Salts - metabolism; Thiazoles - metabolism; tissue repair; water solubility; Wound Healing - drug effects; Wound Healing - physiology
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2013.07.015

Wound healing is a complex process involving many interdependent and overlapping sequences of physiological actions. The application of exogenous lactate released from poly (lactic-co-glycolic acid) (PLGA) polymer accelerated angiogenesis and wound healing processes. Curcumin is a well-known topical wound healing agent for both normal and diabetic-impaired wounds. Hence, we hypothesized that the PLGA nanoparticles encapsulating curcumin could much potentially accelerate the wound healing. In a full thickness excisional wound healing mouse model, PLGA–curcumin nanoparticles showed a twofold higher wound healing activity compared to that of PLGA or curcumin. Histology and RT-PCR studies confirmed that PLGA–curcumin nanoparticles exhibited higher re-epithelialization, granulation tissue formation and anti-inflammatory potential. PLGA nanoparticles offered various benefits for the encapsulated curcumin like protection from light degradation, enhanced water solubility and showed a sustained release of curcumin over a period of 8days. In conclusion, we demonstrated the additive effect of lactic acid from PLGA and encapsulated curcumin for the active healing of wounds. [Display omitted]