Regulatory volume decrease of rat kidney principal cells after successive hypo-osmotic shocks

•Sub-second temporal resolution of RVD in OMCD cells under successive hyposmotic shocks.•After the first hypotonic shock the water permeability of OMCD cells decreases rapidly.•Dynamic modeling of RVD in OMCD cells with fast exchange processes. Outer Medullary Collecting Duct (OMCD) principal cells...

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Published in:Mathematical biosciences 2013-08, Vol.244 (2), p.176-187
Main Authors: Zarogiannis, Sotirios G., Ilyaskin, Alexander V., Baturina, Galina S., Katkova, Liubov E., Medvedev, Dmitriy A., Karpov, Denis I., Ershov, Alexander P., Solenov, Evgeniy I.
Format: Article
Language:English
Subjects:
Animals
Cell Membrane Permeability - physiology
Cell volume regulation
Down-Regulation - physiology
Kidney
Kidney Tubules, Collecting - cytology
Kidney Tubules, Collecting - pathology
Kidney Tubules, Collecting - physiology
Mathematical modeling
Models, Biological
Osmoregulation
Osmotic Pressure - physiology
Osmotic stress
Rats
Time Factors
Water - physiology
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Summary:•Sub-second temporal resolution of RVD in OMCD cells under successive hyposmotic shocks.•After the first hypotonic shock the water permeability of OMCD cells decreases rapidly.•Dynamic modeling of RVD in OMCD cells with fast exchange processes. Outer Medullary Collecting Duct (OMCD) principal cells are exposed to significant changes of the extracellular osmolarity and thus the analysis of their regulatory volume decrease (RVD) function is of great importance in order to avoid cell membrane rupture and subsequent death. In this paper we provide a sub-second temporal analysis of RVD events occurring after two successive hypo-osmotic challenges in rat kidney OMCD principal cells. We performed experimental cell volume measurements and created a mathematical model based on our experimental results. As a consequence of RVD the cell expels part of intracellular osmolytes and reduces the permeability of the plasma membrane to water. The next osmotic challenge does not cause significant RVD if it occurs within a minute after the primary shock. In such a case the cell reacts as an ideal osmometer. Through our model we provide the basis for further detailed studies on RVD dynamical modeling.
ISSN:0025-5564
1879-3134
DOI:10.1016/j.mbs.2013.05.007