Emergence of extended-spectrum β-lactamase-producing Escherichia coli in catheter-associated urinary tract infection in neurogenic bladder patients

• Published in: American journal of infection control 2014-03, Vol.42 (3), p.e29-e31
• Main Authors: Takaba, Kei, BS, Shigemura, Katsumi, MD, PhD, Osawa, Kayo, PhD, Nomi, Masashi, MD, PhD, Fujisawa, Masato, MD, PhD, Arakawa, Soichi, MD, PhD
• Format: Article
• Language: English
• Subjects: beta-Lactamases - secretion; Catheter-associated urinary tract infection (CAUTI); Catheter-Related Infections - diagnosis; Catheter-Related Infections - microbiology; Escherichia coli - classification; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - diagnosis; Escherichia coli Infections - microbiology; Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli; Genotype; Humans; Infection Control; Infectious Disease; Microbial Sensitivity Tests; Molecular Typing; Phenotype; Repetitive-sequence-based polymerase chain reaction (rep-PCR); Urinary Bladder, Neurogenic - complications; Urinary Bladder, Neurogenic - therapy; Urinary Tract Infections - diagnosis; Urinary Tract Infections - microbiology
• ISSN: 0196-6553; 1527-3296
• DOI: 10.1016/j.ajic.2013.11.018

Background Catheter-associated urinary tract infection (CAUTI) is a common clinic problem. The purpose of this study was to investigate recent trends in CAUTI in neurogenic bladder patients focusing on extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Methods Isolates from the urine of neurogenic bladder patients with UTI were investigated. Nine strains of ESBL-producing E coli were assayed by molecular strain typing using the Diversilab system for repetitive-sequence-based polymerase chain reaction (rep-PCR). Results E coli accounted for most of the bacteria (74.1% to 81.0%) that produced ESBLs. Rep-PCR data showed that 7 out of 9 ESBL-producing E coli belonged to the same typing group with high similarity (more than 97% similarity) and that this distribution corresponded with antibiotic resistance patterns. Conclusion ESBL producing E coli strains isolated from CAUTI patients could be discriminated by rep-PCR typing using the Diversilab system in consistent with antibiotic resistance patterns.