Deficient cytokine expression and neutrophil oxidative burst contribute to impaired cutaneous wound healing in diabetic, biofilm-containing chronic wounds

• Published in: Wound repair and regeneration 2013-11, Vol.21 (6), p.833-841
• Main Authors: Nguyen, Khang T., Seth, Akhil K., Hong, Seok J., Geringer, Matthew R., Xie, Ping, Leung, Kai P., Mustoe, Thomas A., Galiano, Robert D.
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Proteins; Biofilms; Chronic Disease; Diabetes Mellitus, Experimental - microbiology; Diabetes Mellitus, Experimental - pathology; Gene Expression Regulation, Bacterial; Interleukin-1beta - metabolism; Male; Mice; Neutrophils - metabolism; Respiratory Burst; Signal Transduction; Staphylococcal Infections - physiopathology; Staphylococcus aureus - isolation & purification; Toll-Like Receptor 2 - metabolism; Toll-Like Receptor 4 - metabolism; Tumor Necrosis Factor-alpha - metabolism; Ulcer - microbiology; Ulcer - pathology; Wound Healing; Wound Infection - microbiology; Wound Infection - physiopathology
• ISSN: 1067-1927; 1524-475X
• DOI: 10.1111/wrr.12109

Diabetic patients exhibit dysregulated inflammatory and immune responses that predispose them to chronic wound infections and the threat of limb loss. The molecular underpinnings responsible for this have not been well elucidated, particularly in the setting of wound biofilms. This study evaluates host responses in biofilm‐impaired wounds using the TallyHo mouse, a clinically relevant polygenic model of type 2 diabetes. No differences in cytokine or Toll‐like receptor (TLR) expression were noted in unwounded skin or noninoculated wounds of diabetic and wild‐type mice. However, diabetic biofilm‐containing wounds had significantly less TLR 2, TLR 4, interleukin‐1β, and tumor necrosis factor‐α expression than wild‐type wounds with biofilm (all p