In vitro antioxidant properties and anthocyanin compositions of elderberry extracts

• Published in: Food chemistry 2014-07, Vol.155, p.112-119
• Main Authors: DUYMUS, Hale Gamze, GÖGER, Fatih, CAN BASER, K. Hüsnü
• Format: Article
• Language: English
• Subjects: Anthocyanins - chemistry; Anthocyanins - isolation & purification; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Biological and medical sciences; Chromatography, High Pressure Liquid; Elderberry; Food toxicology; Fruit - chemistry; HPLC; LC/MS–MS; Mass Spectrometry; Medical sciences; Molecular Structure; Oxidation-Reduction; Phenols - chemistry; Phenols - isolation & purification; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Radical scavenging activity; Sambucus - chemistry; Sambucus nigra; Toxicology
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.01.028

[Display omitted] •The antioxidant activities of elderberry extracts were investigated.•The total phenols and total monomeric anthocyanins were analysed.•Anthocyanin contents of the extracts were characterized by HPLC–UV and LC–MS/MS.•The infusion and 70% ethanol extract were rich in phenolic and anthocyanin levels.•These extracts showed high antiradical activities against both ABTS and DPPH. In this study, dried elderberry fruits growing wild in Turkey were macerated using different solvents and an infusion was prepared according to traditional methods. All extracts were investigated for their total phenolic content, total monomeric anthocyanins, qualitative–quantitative determination of cyanidin-3-glucoside (by HPLC–UV analysis), anthocyanin compositions (by LC/MS–MS), free radical scavenging activity (DPPH and ABTS) and inhibition of β-carotene/linoleic acid co-oxidation. An extract with 70% ethanol was found to be richer in cyanidin-3-glucoside when compared to the other extracts. The infusion was found to be as rich as the 70% ethanol extract. Ethanol and acetone extracts (both 70%) were found to be more active in the free radical activity and β-carotene bleaching assays. Water extract showed good ABTS radical scavenging activity when compared with ascorbic acid.