Fungal pretreatment improves amenability of lignocellulosic material for its saccharification to sugars

• Published in: Carbohydrate polymers 2014, Vol.99, p.264-269
• Main Authors: Deswal, Deepa, Gupta, Rishi, Nandal, Preeti, Kuhad, Ramesh Chander
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Applied sciences; Biological and medical sciences; Brown-rot fungus; Cellulases - chemistry; Cellulose - chemistry; Cellulose - metabolism; Cellulose and derivatives; Coriolaceae - chemistry; Coriolaceae - enzymology; Delignification; Disaccharides - chemistry; Enzymatic saccharification; Exact sciences and technology; Fermentation; Fundamental and applied biological sciences. Psychology; Ganoderma; Ganoderma - chemistry; Ganoderma - enzymology; General agronomy. Plant production; Hydrolysis; Lignin - metabolism; Natural polymers; Physicochemistry of polymers; Pleurotus - chemistry; Pleurotus - enzymology; Pleurotus florida; Saccharum - chemistry; Saccharum - metabolism; Solid-state fermentation; Use of agricultural and forest wastes. Biomass use, bioconversion; White-rot fungi
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.08.045

•Sugarcane bagasse was biologically treated with 3 white-rot fungi.•Shorter incubation resulted in higher H/L ratio.•Myco-substrate showed significant improvement in sugar release on hydrolysis.•Saccharification of lignocellulosic biomass by cellulase from brown-rot fungus. The sugarcane bagasse was biologically pretreated with three white-rot fungi; Pleurotus florida, Coriolopsis caperata RCK 2011 and Ganoderma sp. rckk-02, individually under solid-state fermentation. P. florida, C. caperata RCK 2011 and Ganoderma sp. rckk-02 degraded lignin up to 7.91, 5.48 and 5.58%, respectively. The lignocellulolytic enzymes produced by these fungi were also monitored during solid state fermentation of sugarcane bagasse. The fungal fermented sugarcane bagasse when hydrolyzed with crude cellulases from brown-rot fungus, Fomitopsis sp. RCK2010, released comparatively 1.5–2.4 fold higher sugars than in case of untreated sugarcane bagasse. The study demonstrated that white-rot fungal pretreatment improved the amenability of plant material for enzymatic hydrolysis.