Evaluation of bacteriophage therapy to control Clostridium difficile and toxin production in an in vitro human colon model system

• Published in: Anaerobe 2013-08, Vol.22, p.25-30
• Main Authors: Meader, Emma, Mayer, Melinda J., Steverding, Dietmar, Carding, Simon R., Narbad, Arjan
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - therapeutic use; antibiotics; Bacteria; Bacterial Toxins - antagonists & inhibitors; Bacterial Toxins - biosynthesis; Bacteriophage therapy; bacteriophages; Bacteriophages - drug effects; Bacteriophages - growth & development; Biological Therapy - methods; Cells, Cultured; Clostridioides difficile - metabolism; Clostridioides difficile - pathogenicity; Clostridioides difficile - virology; Clostridium difficile; Clostridium Infections - drug therapy; Clostridium Infections - microbiology; colon; Colon - microbiology; diarrhea; health care workers; Human colon model; Humans; Lysogeny; phage therapy; population; Toxin; Treatment Outcome
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2013.05.001

Clostridium difficile is a leading cause of hospital-acquired diarrhoea and represents a major challenge for healthcare providers. Due to the decreasing efficacy and associated problems of antibiotic therapy there is a need for synergistic and alternative treatments. In this study we investigated the use of a specific bacteriophage, ΦCD27, in a human colon model of C. difficile infection. Our findings demonstrate a significant reduction in the burden of C. difficile cells and toxin production with phage treatment relative to an untreated control, with no detrimental effect on commensal bacterial populations. The results demonstrate the potential of phage therapy, and highlight the limitations of using phages that have lysogenic capacity. •The potential of phage to prevent Clostridium difficile infection has been studied in vitro.•Phage treatment reduced vegetative C. difficile cells in 2 of 3 experiments.•Phage treatment consistently reduced C. difficile toxin production.•Lysogenisation of the phage was demonstrated.•Commensal flora was not negatively affected by phage treatment.