Does the peroxide compound of cytochrome oxidase contain a ferryl iron?

The reaction of peroxide with cytochrome oxidase generates a peroxide compound having a Soret maximum at 428 nm. X-ray absorption spectroscopy analysis of the local structure of the active site iron shows marked similarity to that of the cytochrome c peroxidase intermediate Compound ES, which contai...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-05, Vol.263 (15), p.7159-7163
Main Authors: Kumar, C, Naqui, A, Powers, L, Ching, Y C, Chance, B
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cattle
Copper
cytochrome-c oxidase
Electron Transport Complex IV - metabolism
Enzymes and enzyme inhibitors
Fourier Analysis
Fundamental and applied biological sciences. Psychology
Iron - analysis
Mitochondria, Heart - enzymology
Oxidation-Reduction
Oxidoreductases
peroxides
Peroxides - metabolism
Spectrum Analysis
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Summary:The reaction of peroxide with cytochrome oxidase generates a peroxide compound having a Soret maximum at 428 nm. X-ray absorption spectroscopy analysis of the local structure of the active site iron shows marked similarity to that of the cytochrome c peroxidase intermediate Compound ES, which contains a short iron to proximal nitrogen distance compared to globins. Reductive titration of the 580 nm band of this compound indicates that the iron is one oxidizing equivalent above the resting oxidized form. These results support the presence of a ferryl iron (Fe(IV) = O) in the peroxide compound similar to that found for the peroxidases.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)68620-X