An evaluation of immune system cell infiltrate in the cervical stroma of patients with grade III cervical intraepithelial neoplasia after treatment with intralesional alpha-2B interferon

The aim of this study was to characterize infiltrating immune cells in cervical stroma biopsy samples from patients diagnosed with cervical intraepithelial neoplasias (CINs) who were treated with IFN-alpha 2b. The authors studied 13 volunteers who were diagnosed with Cervical intraepithelial neoplas...

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Bibliographic Details
Published in:European journal of gynaecological oncology 2014, Vol.35 (1), p.20-25
Main Authors: Machado, F A, Abdalla, D R, Montes, L, Etchebehere, R M, Michelin, M A, Murta, E F C
Format: Article
Language:English
Subjects:
Adult
Antigens, CD - immunology
Cervical Intraepithelial Neoplasia - drug therapy
Cervical Intraepithelial Neoplasia - immunology
Cervical Intraepithelial Neoplasia - pathology
Female
Humans
Injections, Intralesional
Interferon-alpha - therapeutic use
Middle Aged
Neoplasm Grading
Prospective Studies
Recombinant Proteins - therapeutic use
Treatment Failure
Uterine Cervical Neoplasms - drug therapy
Uterine Cervical Neoplasms - immunology
Uterine Cervical Neoplasms - pathology
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Summary:The aim of this study was to characterize infiltrating immune cells in cervical stroma biopsy samples from patients diagnosed with cervical intraepithelial neoplasias (CINs) who were treated with IFN-alpha 2b. The authors studied 13 volunteers who were diagnosed with Cervical intraepithelial neoplasia CIN II or III and who received intra-lesional treatment with IFN-alpha 2b. They collected pre- and post-treatment biopsies from each patient. They also examined the slides under a common optical microscope with a X400 lens for biopsy sample sections that were labeled with immunohistochemistry for T lymphocyte, B lymphocyte, natural killer cell, macrophage, iNOS, and perforin markers. The presence of immune response cells in the lesion was observed after treatment with intralesional IFN-alpha 2b in patients with CIN II/III changes, a reduction in CD4+ and CD8+ T lymphocyte infiltration in the women who responded well to treatment. However, there was a significant increase in these markers in samples from women who did not respond to treatment. Nonetheless, immunotherapy with IFN-alpha 2b administered intralesionally in patients with CIN II/III yields favorable results in patients who do not smoke.
ISSN:0392-2936
DOI:10.12892/ejgo23872014