BTG1 expression correlates with the pathogenesis and progression of breast carcinomas

This study aimed to analyze the expression, clinical significance of B cell translocation gene 1 (BTG1) in breast carcinoma and the biological effect in its cell line by BTG1 overexpression. Immunohistochemistry and western blot were used to analyze BTG1 protein expression in 72 cases of breast canc...

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Bibliographic Details
Published in:Tumor biology 2014-04, Vol.35 (4), p.3317-3326
Main Authors: Sheng, S. H., Zhao, C. M., Sun, G. G.
Format: Article
Language:English
Subjects:
Adult
Aged
Biomedical and Life Sciences
Biomedicine
Breast - chemistry
Breast cancer
Breast Neoplasms - etiology
Breast Neoplasms - pathology
Cancer Research
Cell Proliferation
Disease Progression
Female
G1 Phase
Humans
Matrix Metalloproteinase 9 - analysis
Metastasis
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Proteins - analysis
Neoplasm Proteins - genetics
Neoplasm Proteins - physiology
Pathogenesis
Prognosis
Protein expression
Research Article
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Summary:This study aimed to analyze the expression, clinical significance of B cell translocation gene 1 (BTG1) in breast carcinoma and the biological effect in its cell line by BTG1 overexpression. Immunohistochemistry and western blot were used to analyze BTG1 protein expression in 72 cases of breast cancer and 36 cases of normal tissues to study the relationship between BTG1 expression and clinical factors. Recombinant lentiviral vector was constructed to over-express EMP-1 and then infect breast cancer MCF-7 cell line. Quantitative real-time RT-PCR (qRT-PCR) and western blot were used to detect the mRNA level and protein of BTG1. MTT assay, cell apoptosis, cell cycles, migration and invasion assays were also conducted as to the influence of the upregulated expression of BTG1 that might be found on MCF-7 cells biological effect. The level of BTG1 protein expression was found to be significantly lower in breast cancer tissue than normal tissues ( P  
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-013-1437-0