Phenazine antibiotic production and antifungal activity are regulated by multiple quorum-sensing systems in Pseudomonas chlororaphis subsp. aurantiaca StFRB508

• Published in: Journal of bioscience and bioengineering 2013-11, Vol.116 (5), p.580-584
• Main Authors: Morohoshi, Tomohiro, Wang, Wen-Zhao, Suto, Tomonori, Saito, Yuki, Ito, Satoshi, Someya, Nobutaka, Ikeda, Tsukasa
• Format: Article
• Language: English
• Subjects: 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - biosynthesis; 4-Butyrolactone - chemistry; 4-Butyrolactone - pharmacology; Acylhomoserine lactone; Anti-Bacterial Agents - biosynthesis; Antifungal activity; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biotechnology; Computer Simulation; Fundamental and applied biological sciences. Psychology; Fusarium - growth & development; Fusarium oxysporum; Genome, Bacterial - genetics; Phenazine; Phenazines - metabolism; Pseudomonas; Pseudomonas - drug effects; Pseudomonas - genetics; Pseudomonas - metabolism; Pseudomonas chlororaphis; Quorum sensing; Quorum Sensing - drug effects; Quorum Sensing - physiology
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2013.04.022

A number of gram-negative bacteria have a quorum-sensing system and produce the N-acylhomoserine lactone (AHL) as a signal molecule. Pseudomonas chlororaphis subsp. aurantiaca StFRB508 produces one of the phenazine derivatives, phenazine-1-carboxylic acid (PCA). Whole-genome sequencing of StFRB508 revealed the presence of two sets of AHL-synthase and AHL-receptor gene, phzIR and aurIR. The mutation of phzI drastically decreased PCA production, but the mutation of aurI did not affect PCA production. The phzI and aurI double mutant did not show any PCA production. StFRB508 produces three major AHLs, N-butyryl-l-homoserine lactone (C4-HSL), N-hexanoyl-l-homoserine lactone (C6-HSL), and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). As the results of TLC analysis, PhzI mainly catalyzes the biosynthesis of 3-hydroxy-C6-HSL, and AurI catalyzes the biosynthesis of C4-HSL and C6-HSL. PCA production in the phzI and aurI double mutant was restored by exogenous AHLs and the most active AHL was 3-hydroxy-C6-HSL. StFRB508 showed high inhibitory activity of the development of mycelia of plant pathogenic fungi, Fusarium oxysporum f. sp. conglutinans. However, the phzI and aurI double mutant could not inhibit the development of mycelia. These results demonstrated that the multiple quorum-sensing system play an important role in PCA production and antifungal activity in StFRB508.