Community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees

• Published in: Applied microbiology and biotechnology 2014-02, Vol.98 (3), p.1449-1458
• Main Authors: Yang, Jiang-Ke, Zhang, Jing-Jing, Yu, Heng-Yu, Cheng, Jian-Wen, Miao, Li-Hong
• Format: Article
• Language: English
• Subjects: Alternative energy; Bacillus cereus; Bacillus subtilis; Bacteria; Bacteria - classification; Bacteria - enzymology; Bacteria - genetics; Bacteria - isolation & purification; Biodiesel fuels; Biofuels; Biomass; Biomedical and Life Sciences; Biota; Biotechnology; broadleaved evergreens; Carbon; Carbon sources; Cellulase; Cellulase - metabolism; cellulolytic microorganisms; Cellulose; China; Chryseobacterium; Chryseobacterium indoltheticum; Cluster Analysis; Community composition; community structure; Conversion; Deciduous trees; Decomposition; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; endo-1,4-beta-glucanase; Environmental aspects; Environmental Biotechnology; Enzymes; Ethanol; Evergreen trees; Flavobacterium; Flavobacterium pectinovorum; Forest soils; Forests; genes; Genetic aspects; Life Sciences; Microbacterium; Microbacterium testaceum; Microbial Genetics and Genomics; Microbiology; Microorganisms; Molecular Sequence Data; nitrogen content; nucleotide sequences; Nutrient balance; Nutrient sources; Organic carbon; Paenibacillus; Phylogenetics; Phylogeny; Physiological aspects; Plant species; planting; Polymorphism; Polymorphism, Restriction Fragment Length; Population density; Pseudomonas; restriction fragment length polymorphism; ribosomal RNA; RNA, Ribosomal, 16S - genetics; sequence analysis; Sequence Analysis, DNA; Soil fertility; Soil Microbiology; Soil nutrients; Soil properties; Studies; trees
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-013-5130-4

Cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. These bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. In this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (Chang Qing Garden, CQG) and broad-leaved evergreen (Forest Park, FP) trees in Wuhan, China were resolved through restriction fragment length polymorphism (RFLP) and sequencing analysis of the 16S rRNA gene. All of the isolates exhibited 35 RFLP fingerprint patterns and were clustered into six groups at a similarity level of 50 %. The phylogeny analysis based on the 16S rRNA gene sequence revealed that these RFLP groups could be clustered into three phylogenetic groups and further divided into six subgroups at a higher resolution. Group I consists of isolates from Bacillus cereus, Bacillus subtilis complex (I-A) and from Paenibacillus amylolyticus-related complex (I-B) and exhibited the highest cellulase activity among all of the cellulolytic bacteria isolates. Cluster II consists of isolates belonging to Microbacterium testaceum (II-A), Chryseobacterium indoltheticum (II-B), and Flavobacterium pectinovorum and the related complex (II-C). Cluster III consists of isolates belonging to Pseudomonas putida-related species. The community shift with respect to the plant species and the soil properties was evidenced by the phylogenetic composition of the communities. Groups I-A and I-B, which account for 36.0 % of the cellulolytic communities in the CQG site, are the dominant groups (88.4 %) in the FP site. Alternatively, the ratio of the bacteria belonging to group III (P. putida-related isolates) shifted from 28.0 % in CQG to 4.0 % in FP. The soil nutrient analysis revealed that the CQG site planted with deciduous broad-leaved trees has a richer organic nutrient (total organic carbon and total nitrogen) than the FP site planted with evergreen broad-leaved trees. Against this background, the population density and the diversity of cellulolytic bacteria in the CQG site are clearly higher than those in the FP site, and the latter was dominated with high-cellulase-activity Bacillus- and Paenibacillus-related bacteria. The canonical correspondence analysis further indicated that the distribution of these groups is correlated w