Glutathione-s-transferase modified electrodes for detecting anticancer drugs

With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal....

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Bibliographic Details
Published in:Biosensors & bioelectronics 2014-08, Vol.58, p.232-236
Main Authors: Materon, Elsa M., Jimmy Huang, Po-Jung, Wong, Ademar, Pupim Ferreira, Antonio A., Sotomayor, Maria Del Pilar Taboada, Liu, Juewen
Format: Article
Language:English
Subjects:
Antineoplastic Agents - analysis
Antineoplastic Agents - chemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
Carbon past electrode
Cisplatin
Colorimetry - instrumentation
Conductometry - instrumentation
Drug Evaluation, Preclinical - instrumentation
Enzymes, Immobilized - chemistry
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Glutathione
Glutathione Transferase - chemistry
Glutathione-s-transferase
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Summary:With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal. In particular, it is challenging to have a general method to monitor various types of anticancer drugs with different structures. In this work, a biosensor was developed to detect anticancer drugs by modifying carbon paste electrodes with glutathione-s-transferase (GST) enzymes. GST is widely studied in the metabolism of xenobiotics and is a major contributing factor in resistance to anticancer drugs. The measurement of anticancer drugs is based on competition between 1-chloro-2,4-dinitrobenzene (CDNB) and the drugs for the GST enzyme in the electrochemical potential at 0.1V vs. Ag/AgCl by square wave voltammetry (SWV) or using a colorimetric method. The sensor shows a detection limit of 8.8μM cisplatin and exhibits relatively long life time in daily measurements. •Glutathione-s-transferase (GST) as a general probe for anticancer drugs.•Both optical and electrochemical detection demonstrated.•Many anticancer drugs are tested as substrate and target for GST.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.02.070