Co-administration of flagellin augments immune responses to inactivated foot-and-mouth disease virus (FMDV) antigen

Foot-and-mouth disease virus (FMDV) is one of the most contagious animal virus known that affects livestock health and production. This study aimed to investigate the effect of flagellin, a toll-like receptor 5 agonist, on the immune responses to inactivated FMDV antigen in guinea pig model. Our res...

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Bibliographic Details
Published in:Research in veterinary science 2013-12, Vol.95 (3), p.936-941
Main Authors: Hajam, Irshad A., Dar, Pervaiz A., ChandraSekar, Shanmugam, Nanda, Raj Kishore, Kishore, Subodh, Bhanuprakash, Veerakyathappa, Ganesh, Kondabattula
Format: Article
Language:English
Subjects:
agonists
animal viruses
Animals
Antibodies, Neutralizing - immunology
Antigens, Viral - immunology
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Flagellin
Flagellin - immunology
Flagellin - pharmacology
Foot & mouth disease
Foot-and-mouth disease virus
Foot-and-Mouth Disease Virus - immunology
Guinea pig
Guinea Pigs
Histocompatibility Antigens Class II - immunology
Hogs
Immune response
Immune system
livestock
Male
Molecular adjuvant
Neutralization Tests - veterinary
neutralizing antibodies
Recombinant Proteins - immunology
Vaccines
Veterinary medicine
viral antigens
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Summary:Foot-and-mouth disease virus (FMDV) is one of the most contagious animal virus known that affects livestock health and production. This study aimed to investigate the effect of flagellin, a toll-like receptor 5 agonist, on the immune responses to inactivated FMDV antigen in guinea pig model. Our results showed that the co-administration of flagellin with FMDV antigen through intradermal route induces earlier and higher anti-FMDV neutralizing antibody responses as compared to FMDV antigen alone. Both IgG1 and IgG2 antibody-isotype responses were enhanced, but the IgG1/IgG2 ratios were relatively low, indicative of TH1 type of immune activation. On live viral challenge, flagellin+FMDV immunized guinea pigs showed 70% (7 out of 10) protection rate as compared to 40% (4 out of 10) in FMDV alone immunized guinea pigs. The results demonstrate that the co-administration of flagellin augments immune responses (preferably TH1 type) and protective efficacy against FMDV in guinea pigs.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2013.07.021