Evaluation of a lytic bacteriophage, Φ st1, for biocontrol of Salmonella enterica serovar Typhimurium in chickens

In this study, a Salmonella Typhimurium lytic bacteriophage, Φ st1, which was isolated from chicken faecal material, was evaluated as a candidate for biocontrol of Salmonella in chickens. The morphology of Φ st1 showed strong resemblance to members of the Siphoviridae family. Φ st1 was observed to b...

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Bibliographic Details
Published in:International journal of food microbiology 2014-02, Vol.172, p.92-101
Main Authors: Wong, Chuan Loo, Sieo, Chin Chin, Tan, Wen Siang, Abdullah, Norhani, Hair-Bejo, Mohd, Abu, Jalila, Ho, Yin Wan
Format: Article
Language:English
Subjects:
Animal Husbandry - methods
Animals
Bacteria
Bacterial Load
Bacteriophage
Bacteriophages - genetics
Bacteriophages - physiology
Bacteriophages - ultrastructure
Biological and medical sciences
Biological Control Agents
Cecum - microbiology
Chicken
Chickens
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Genome, Viral
Host-Pathogen Interactions
Hydrogen-Ion Concentration
Phage therapy
Poultry Diseases - prevention & control
Salmonella Infections, Animal - microbiology
Salmonella Infections, Animal - prevention & control
Salmonella Typhimurium
Salmonella typhimurium - virology
Salmonellosis
Siphoviridae
Temperature
Time
Virus Replication
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Summary:In this study, a Salmonella Typhimurium lytic bacteriophage, Φ st1, which was isolated from chicken faecal material, was evaluated as a candidate for biocontrol of Salmonella in chickens. The morphology of Φ st1 showed strong resemblance to members of the Siphoviridae family. Φ st1 was observed to be a DNA phage with an estimated genome size of 121kbp. It was found to be able to infect S. Typhimurium and S. Hadar, with a stronger lytic activity against the former. Subsequent characterisation of Φ st1 against S. Typhimurium showed that Φ st1 has a latent period of 40min with an average burst size of 22 particles per infective centre. Approximately 86.1% of the phage adsorbed to the host cells within the initial 5min of infection. At the optimum multiplicity of infection (MOI) (0.1), the highest reduction rate of S. Typhimurium (6.6log10CFU/ml) and increment in phage titre (3.8log10PFU/ml) was observed. Φ st1 produced adsorption rates of 88.4–92.2% at pH7–9 and demonstrated the highest bacteria reduction (6.6log10CFU/ml) at pH9. Φ st1 also showed an insignificant different (P>0.05) reduction rate of host cells at 37°C (6.4log10CFU/ml) and 42°C (6.0log10CFU/ml). The in vivo study using Φ st1 showed that intracloacal inoculation of ~1012PFU/ml of the phage in the chickens challenged with ~1010CFU/ml of S. Typhimurium was able to reduce (P
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2013.11.034