Repeated supra-maximal sprint cycling with and without sodium bicarbonate supplementation induces endothelial microparticle release

• Published in: European journal of sport science 2014, Vol.14 (4), p.345-352
• Main Authors: Kirk, Richard J., Peart, Daniel J., Madden, Leigh A., Vince, Rebecca V.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; anaerobic exercise; Antigens, CD - blood; Bicycling - physiology; Cell-Derived Microparticles - metabolism; Dietary Supplements; Double-Blind Method; Endoglin; Endothelial Cells - drug effects; Endothelial function; Flow Cytometry; Humans; Male; microparticles; Receptors, Cell Surface - blood; sodium bicarbonate; Sodium Bicarbonate - pharmacology; vascular cell adhesion molecule-1; Vascular Cell Adhesion Molecule-1 - blood; Young Adult
• ISSN: 1746-1391; 1536-7290
• DOI: 10.1080/17461391.2013.785600

Under normal homeostatic conditions, the endothelium releases microparticles (MPs), which are known to increase under stressful conditions and in disease states. CD105 (endoglin) and CD106 (vascular cell adhesion molecule-1) are expressed on the surface of endothelial cells and increased expression in response to stress may be observed. A randomised-controlled double-blinded study aimed to examine the use of endothelial MPs as a marker for the state of one's endothelium, as well as whether maintaining acid-base homeostasis affects the release of these MPs. This study tested seven healthy male volunteers, who completed a strenuous cycling protocol, with venous blood analysed for CD105+ and CD106+ MPs by flow cytometry at regular intervals. Prior to each trial participants consumed either 0.3 g·kg −1 body mass of sodium bicarbonate (NaHCO 3 ), or 0.045 g·kg −1 body mass of sodium chloride (NaCl). A significant rise in endothelial CD105+ MPs and CD106+ MPs (p