Evaluation of Genetic Diversity of Raya (Brassica juncea) through RAPD Markers

• Published in: International journal of agriculture and biology 2013-12, Vol.15 (6)
• Main Authors: Riffat Tahira, Ihsan-Ullah, Mahjabeen Saleem
• Format: Article
• Language: English
• Subjects: Brassica juncea
• ISSN: 1560-8530; 1814-9596

This study was designed to explore the genetic relatedness among 30 Brassica juncea lines and varieties included in "development of canola varieties in B. juncea" program, using Randomly Amplified Polymorphic DNA (RAPD) markers. All used RAPD markers produced a total of 104 loci with an average of 8.6 bands per primer. The range of amplified bands was between 300bp to 3kb. The largest fragment of 3kb was amplified with three primers, A-5, A-19 and B-05. On an average 84% similarity matrix (SM) was observed between all thirty genotypes. The most diverse genotype was RBJ-97001 with average genetic diversity of 29% while RBJ-02017 showed the highest average genetic SM (87%) to all other genotypes. The genotypes RBJ-07017 and RL-18 were observed most similar to each other with genetic SM of 95%, whereas, Raya 49/2 and RBJ-97001 were found the most diverse lines with genetic SM of 71%. Unweighed pair group method of arithmetic means cluster analysis indicated that 30 genotypes were capable of being classified into two major groups, A and B. Both groups were further divided into two sub-groups. The information obtained on genetic distances will be useful in future breeding of Raya cultivars for the development of canola type varieties along with germplasm characterization. copyright 2013 Friends Science Publishers