Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation

Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment....

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Bibliographic Details
Published in:Transfusion and apheresis science 2014-06, Vol.50 (3), p.443-450
Main Authors: D’Rozario, James, Parisotto, Robin, Stapleton, Jennifer, Gidley, Alison, Owen, David
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Amyloidosis - therapy
Antigens, CD34
Autografts
Autologous transplantation
CD34+ cell count
Cryopreservation
Female
Graft Survival
Health technology assessment
Hematology, Oncology and Palliative Medicine
Hematopoietic Stem Cells
Humans
Male
Middle Aged
Multiple Sclerosis - therapy
Neoplasms - therapy
Neutrophil engraftment
Neutrophils
Peripheral Blood Stem Cell Transplantation
Platelet engraftment
Retrospective Studies
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Summary:Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment. Viable CD34+ cell numbers are enumerated in most laboratories using the ISHAGE single platform flow cytometric method which utilizes monoclonal antibodies to CD45, CD34 and 7 amino actinomycin D (7AAD) dye exclusion. Methods One hundred and six consecutive autologous transplantation procedures underwent viable CD34+ cell enumeration at the time of harvesting and post thaw prior to re-infusion. Neutrophil and platelet engraftment and markers of haematopoietic support were analyzed. Results Mean pre-cryopreservation viable CD34+ numbers were 4.882 × 106 /kg. Mean post thaw viable CD34+ numbers were 3.234 × 106 /kg. Mean loss of viable CD34+ cells with processing and cryo-preservation was 1.648 × 106 /kg (33%). For neutrophil engraftment, there was no significant difference between high (⩾3.0 × 106 /kg) and low (
ISSN:1473-0502
1878-1683
DOI:10.1016/j.transci.2014.02.021