GTP Binding Leads to Narrowing of the Conformer Population While Preserving the Structure of the RNA Aptamer: A Site-Specific Time-Resolved Fluorescence Dynamics Study

• Published in: Biochemistry (Easton) 2012-11, Vol.51 (46), p.9260-9269
• Main Authors: Singh, T. Sanjoy, Rao, B. J, Krishnamoorthy, G
• Format: Article
• Language: English
• Subjects: Aptamers, Nucleotide - chemistry; Guanosine Triphosphate - chemistry; Kinetics; Nucleic Acid Conformation; RNA - chemistry; Spectrometry, Fluorescence
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi301110u

In this study, we employed a combination of steady-state and time-resolved fluorescence spectroscopy and studied the site-specific dynamics in a GTP aptamer using 2-aminopurine as a fluorescent probe. We compared the dynamics of the GTP-bound aptamer with that of the free aptamer as well as when it is denatured. GTP binding leads to an overall compaction of structure in the aptamer. The general pattern of fluorescence lifetimes and correlation times scanned across several locations in the aptamer does not seem to change following GTP binding. However, a remarkable narrowing of the lifetime distribution of the aptamer ensues following its compaction by GTP binding. Interestingly, such a “conformational narrowing” is evident from the lifetime readouts of the nucleotide belonging to the stem as well as the “bulge” part of the aptamer, independent of whether it is directly interacting with GTP. Taken together, these results underscore the importance of an overall intrinsic structure associated with the free aptamer that is further modulated following GTP binding. This work provides strong support for the “conformational selection” hypothesis of ligand binding.