Genetic and biochemical studies of the highly active esterases A' and B associated with organophosphate resistance in mosquitoes of the Culex pipiens complex

The highly active esterases A' and B that cannot be dissociated from OP resistance in Culex pipiens from France and California are shown to have equivalent Km values (2.1 x 10(-6) M/min/mosquito) but different turnover rates (Vm = 2.13 and 0.57 x 10(-6) M/min/mosquito, respectively) and pH for...

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Bibliographic Details
Published in:Biochemical genetics 1981-10, Vol.19 (9-10), p.909-919
Main Authors: Pasteur, N, Iseki, A, Georghiou, G P
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Crosses, Genetic
Culex pipiens
Culicidae - genetics
enzymatic activity
esterase
Esterases - genetics
Female
gene mapping
Genes
genetic control
Genetic Linkage
Insecticide Resistance
Insecticides - pharmacology
kinetics
Male
Organophosphorus Compounds
pesticide resistance
pesticides (orthophosphate)
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Summary:The highly active esterases A' and B that cannot be dissociated from OP resistance in Culex pipiens from France and California are shown to have equivalent Km values (2.1 x 10(-6) M/min/mosquito) but different turnover rates (Vm = 2.13 and 0.57 x 10(-6) M/min/mosquito, respectively) and pH for maximum activity. Both enzymes have broad substrate specificities and at least one, esterase A', can hydrolyze OP insecticides. In addition, esterases A' and B are coded by two closely linked genes, Est-3 and Est-2, respectively (0.67 unit of crossing over), located on the same autosome as pl, a locus attributed to linkage group III. The estimated distance between Est-2 and pl was 9.4 units.
ISSN:0006-2928
1573-4927
DOI:10.1007/BF00504256