Desmin and nerve terminal expression during embryonic development of the lateral pterygoid muscle in mice

• Published in: Archives of oral biology 2014-09, Vol.59 (9), p.871-879
• Main Authors: Yamamoto, Masahito, Shinomiya, Takashi, Kishi, Asuka, Yamane, Shigeki, Umezawa, Takashi, Ide, Yoshinobu, Abe, Shinichi
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Animals; Dentistry; Desmin; Desmin - metabolism; Dissection; Embryo; Female; Immunohistochemistry; Lateral pterygoid; Mice; Mouse; Muscle Development - physiology; Nerve terminal; Pregnancy; Pterygoid Muscles - embryology; Pterygoid Muscles - innervation; Pterygoid Muscles - metabolism; Reverse Transcriptase Polymerase Chain Reaction
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2014.03.011

Abstract Objective In adults, the lateral pterygoid muscle (LPM) is usually divided into the upper and lower head, between which the buccal nerve passes. Recent investigations have demonstrated foetal developmental changes in the topographical relationship between the human LPM and buccal nerve. However, as few studies have investigated this issue, we clarified the expression of desmin and nerve terminal distribution during embryonic development of the LPM in mice. Methods We utilized immunohistochemical staining and reverse transcription chain reaction (RT-PCR) to clarify the expression of desmin and nerve terminal distribution. Results We observed weak expression of desmin in the LPM at embryonic day (ED) 11, followed by an increase in expression from embryonic days 12–15. In addition, starting at ED 12, we observed preferential accumulation of desmin in the vicinity of the myotendinous junction, a trend that did not change up to ED 15. Nerve terminal first appeared at ED 13 and formed regularly spaced linear arrays at the centre of the muscle fibre by ED 15. The results of immunohistochemical staining agreed with those of RT-PCR analysis. Conclusion We found that desmin accumulated in the vicinity of the myotendinous junction starting at ED 12, prior to the onset of jaw movement. We speculate that the accumulation of desmin is due to factors other than mechanical stress experienced during early muscle contraction. Meanwhile, the time point at which nerve terminals first appeared roughly coincided with the onset of jaw movement.