Integration of exogenous DNA into the genome of Azotobacter vinelandii

The soil bacterium Azotobacter vinelandii was genetically transformed by chromosomal integration to ampicillin and/or tetracycline resistance using restriction endonuclease-linearized plasmids. Polyacrylamide gel electrophoresis of protein extracts from three independently isolated ampicillin resist...

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Bibliographic Details
Published in:Archives of microbiology 1989-10, Vol.152 (5), p.437-440
Main Authors: Renaud, C.S, Pasternak, J.J, Glick, B.R
Format: Article
Language:English
Subjects:
ADN
AZOTOBACTER
Bakterien
beta -lactamase
Desoxyribonukleinsaeure
DNA
DNA-Plasmid
genes
GENETIC ENGINEERING
GENETIC TRANSFORMATION
GENIE GENETIQUE
GENOMAS
GENOME
GENOMES
INGENIERIA GENETICA
plasmids
TRANSFORMACION GENETICA
transformation
Transformation (Genetik)
TRANSFORMATION GENETIQUE
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Summary:The soil bacterium Azotobacter vinelandii was genetically transformed by chromosomal integration to ampicillin and/or tetracycline resistance using restriction endonuclease-linearized plasmids. Polyacrylamide gel electrophoresis of protein extracts from three independently isolated ampicillin resistant transformants showed the presence of a 28 Kd band which is the approximate size of the ampicillin resistance gene product (i.e., beta -lactamase). DNA hybridization showed that the chromosomal DNA from transformed cells contained plasmid DNA sequences at discrete sites.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00446925