Simultaneous diagnostic platform of genotyping EGFR, KRAS, and ALK in 510 Korean patients with non-small-cell lung cancer highlights significantly higher ALK rearrangement rate in advanced stage

Background Simultaneous genotyping has advantages in turnaround time and detecting the real mutational prevalence in unresectable non‐small‐cell lung cancer (NSCLC), a group not previously genetically characterized. Methods We developed simultaneous panel of screening EGFR and KRAS mutations by dire...

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Published in:Journal of surgical oncology 2014-09, Vol.110 (3), p.245-251
Main Authors: Kim, Tae-Jung, Park, Chan Kwon, Yeo, Chang Dong, Park, Kihoon, Rhee, Chin Kook, Kim, Jusang, Kim, Seung Joon, Lee, Sang Haak, Lee, Kyo-Young, Yoon, Hyoung-Kyu
Format: Article
Language:English
Subjects:
Adult
Age Factors
Aged
Aged, 80 and over
ALK
Asian Continental Ancestry Group - genetics
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - pathology
EGFR
Female
fluorescence in situ hybridization
Gene Rearrangement
Genotype
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
KRAS
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Male
Middle Aged
Mutation
non-small-cell lung carcinoma
Polymerase Chain Reaction
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins p21(ras)
ras Proteins - genetics
Receptor Protein-Tyrosine Kinases - genetics
Receptor, Epidermal Growth Factor - genetics
Republic of Korea
Sex Factors
Smoking - epidemiology
Young Adult
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Summary:Background Simultaneous genotyping has advantages in turnaround time and detecting the real mutational prevalence in unresectable non‐small‐cell lung cancer (NSCLC), a group not previously genetically characterized. Methods We developed simultaneous panel of screening EGFR and KRAS mutations by direct sequencing or PNA clamping, and ALK rearrangement by fluorescent in situ hybridization (FISH) in multicenter manner. Results Of 510 NSCLC Korean patients, simultaneous genotyping identified mutations of EGFR (29.0%) and KRAS (8.6%) and rearrangement of ALK (9.2%). Seven patients had overlaps in mutations. Although several well‐known associations between genotypes and clinical characteristics were identified, we found no relationship between ALK rearrangement and sex or smoking history. Unlike the other genotype mutations, ALK rearrangement was associated with advanced disease. Among the ALK‐negative group, patients with 10–15% of ALK FISH split shared characteristics, such as younger age and advanced stage disease, more with the ALK‐positive group (>15% ALK FISH split) than
ISSN:0022-4790
1096-9098
DOI:10.1002/jso.23646