Prevention of mold growth and toxin production through control of environmental conditions [Mycotoxins, fungi, agricultural products]

• Published in: Journal of food protection 1982-04, Vol.45 (6), p.519-526
• Main Authors: Northolt, Martin D, Bullerman, Lloyd B
• Format: Article
• Language: English
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028x-45.6.519

Environmental conditions influence mold growth and mycotoxin production. Such things as water activity (a ), temperature, pH and atmosphere can strongly affect and profoundly alter patterns of growth and mycotoxin production. Generally, maintenance of low temperatures will prevent aflatoxin production in stored products, whereas other toxins such as penicillic acid, patulin, zearolenone and T-2 toxin may be produced at low temperatures. Toxic Penicillium and Fusarium species are generally more capable of growth at low temperatures than are toxic species of Aspergillus . Temperature interacts with a to influence mold growth and mycotoxin production. Aflatoxin B can be produced at conditions of a and temperature which are close to the minimum a and temperature for growth. On the other hand, patulin, penicillic acid and ochratoxin A are produced within a narrower range of a and temperature, compared with those for growth. In fact, production of patulin and penicillic acid by Penicillium species appears to be confined to high a values only. In optimal substrates, the minima of a and temperature for growth and toxin production may be lower than in other substrates. It appears that pH and substrate composition have no great effect on growth of toxic molds, but may have a great influence on toxin production. Presence of CO and O influences mold growth and mycotoxin production. A 20% level of CO in air depresses aflatoxin production and markedly depresses mold growth. Decreasing the O concentration of air to 10% depresses aflatoxin production, but only at O levels of less than 1% are growth and aflatoxin production completely inhibited. With patulin- and sterigmatocystin-producing molds, concentrations of 40% CO depress growth and toxin production, but a level of 90% CO is needed to completely inhibit production of these toxins. Decreasing O concentration to 2% depresses production of patulin and sterigmatocystin but does not affect fungal growth. Only at levels down to 0.2% are growth and toxin production completely inhibited. Controlled atmospheres with increased CO (above 10%) and decreased O (2%) can be used to retard mold growth. Exclusion of O by vacuum packaging in materials with low O permeability will depress or even prevent aflatoxin production. Presence of other microorganisms may also restrict fungal growth and mycotoxin production. Aflatoxin production by Aspergillus flavus in mixed cultures with Aspergillus niger is less than in pure culture. Mixture