DNA repair synthesis in individuals with and without a family history of cancer

The influence of family history on DNA repair synthesis, unscheduled DNA synthesis (UDS), was assessed in volunteers with or without a family history of cancer. UDS, following treatment of mononuclear leukocytes with N-acetoxy-2-acetylaminofluorene, was measured as the incorporation of [3H] into DNA...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1989-04, Vol.10 (4), p.693-697
Main Authors: Pero, Ronald W., Johnson, Desmond B., Markowitz, Melvin, Doyle, Geraldine, Lund-Pero, Margaretha, Seidegard, Janeric, Halper, Marilyn, Miller, Daniel G.
Format: Article
Language:English
Subjects:
Acetoxyacetylaminofluorene - pharmacology
Age Factors
Aged
Biological and medical sciences
DNA - biosynthesis
DNA Repair
Female
General aspects
Humans
Leukocytes, Mononuclear - metabolism
Male
Medical sciences
Middle Aged
Neoplasms - genetics
Organ Specificity
Smoking
Tumors
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Summary:The influence of family history on DNA repair synthesis, unscheduled DNA synthesis (UDS), was assessed in volunteers with or without a family history of cancer. UDS, following treatment of mononuclear leukocytes with N-acetoxy-2-acetylaminofluorene, was measured as the incorporation of [3H] into DNA in the presence of hydroxyurea. The positive family history group (n=71) had an average of 2.4 first-degree relatives with cancer, defined as any major cancer, excluding skin cancer: 31 participants reported that cancer occurred in both their parents. The ‘no family history’ comparison group (n=29) had no family history of cancer through the second degree. There was a significant reduction in UDS in cells from individuals with family history, compared to those with no family history (P>0.002). This relationship was not explained by factors known to influence UDS, such as age, smoking or hypertension. We conclude that reduced UDS in mononuclear leukocytes is associated with a family history of any major cancer, and is not confined to a history of cancer of any single organ site. This conclusion is further supported by the observation that individuals (n=13) with parents who had an earlier onset of cancer (60 years).
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/10.4.693