Age-related activation of MKK/p38/NF-κB signaling pathway in lung: From mouse to human

• Published in: Experimental gerontology 2014-09, Vol.57, p.29-40
• Main Authors: Ren, Xiaoxia, Du, Huadong, Li, Yan, Yao, Xiujuan, Huang, Junmin, Li, Zongli, Wang, Wei, Li, Junfa, Han, Song, Wang, Chen, Huang, Kewu
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aging - metabolism; Aging - pathology; Animals; beta-Galactosidase - metabolism; Cytokines; Cytokines - metabolism; Humans; Inflammaging; Lipopolysaccharide; Lipopolysaccharides; Lung; Lung - metabolism; Lung - pathology; Male; MAP Kinase Signaling System; Mice, Inbred C57BL; Middle Aged; NF-kappa B - metabolism; P38; Stress, Physiological
• ISSN: 0531-5565; 1873-6815
• DOI: 10.1016/j.exger.2014.04.017

We and others previously reported that the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 significantly accumulate with age in mouse lung. This is accompanied by elevated phosphorylation of p38. Here, we further investigate whether aging affects activation of p38 signaling and the inflammatory reaction after exposure to lipopolysaccharide (LPS) in the lungs of mice in vivo and humans ex vivo. The data showed that activation of p38 peaked at 0.5h and then rapidly declined in young (2-month-old) mouse lung, after intranasal inhalation challenge with LPS. In contract, activation of p38 peaked at 24h and was sustained longer in aged (20-month-old) mice. As well as altered p38, activations of its upstream activator MKK and downstream substrate NF-κB were also changed in the lungs of aged mice, which corresponded with the absence in the early phase but delayed increases in concentrations of TNF-α, IL-1β and IL-6. Consistent with the above observations in mice, similar patterns of p38 signaling also occurred in human lungs. Compared with younger lungs from adult–middle aged subjects, the activation of p38, MKK and NF-κB, as well as the production of pro-inflammatory cytokines were significantly increased in the lungs of older subjects ex vivo. Exposure of human lung cells to LPS induced rapid activation of p38, MKK and NF-κB in these cells from adult–middle aged subjects, but not older subjects, with increases in the production of the pro-inflammatory cytokines. The LPS-induced rapid activation in the lung cells from adult–middle aged subjects occurred as early as 0.25h after exposure, and then declined. Compared with adult–middle aged subjects, the LPS exposure did not induce marked changes in the early phase, either in the activation of p38, MKK and NF-κB, or in the production of TNF-α, IL-1β or IL-6 in the lung cells from older subjects. In contrast, these changes occurred relatively late, peaked at 16h and were sustained longer in the lungs of older subjects. These data support the hypothesis that the sustained activation of the p38 signaling pathway at baseline and the absence in the early phase but delayed of p38 signaling pathway response to LPS in the elderly may play important roles in increased susceptibility of aged lungs to inflammatory injury. •The basal activation of the p38 signaling is increased in aged lungs.•The activation of p38 is absent in aged lungs after LPS exposure in the early phase.•The activa