Comparison of Biofilm Formation between Major Clonal Lineages of Methicillin Resistant Staphylococcus aureus: e104561

Objectives Epidemic methicillin-resistant S. aureus (MRSA) clones cause infections in both hospital and community settings. As a biofilm phenotype further facilitates evasion of the host immune system and antibiotics, we compared the biofilm-forming capacities of various MRSA clones. Methods Seventy...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8)
Main Authors: Vanhommerig, Evelyn, Moons, Pieter, Pirici, Daniel, Lammens, Christine, Hernalsteens, Jean-Pierre, Greve, Henri De, Kumar-Singh, Samir, Goossens, Herman, Malhotra-Kumar, Surbhi
Format: Article
Language:English
Subjects:
Staphylococcus aureus
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Summary:Objectives Epidemic methicillin-resistant S. aureus (MRSA) clones cause infections in both hospital and community settings. As a biofilm phenotype further facilitates evasion of the host immune system and antibiotics, we compared the biofilm-forming capacities of various MRSA clones. Methods Seventy-six MRSA classified into 13 clones (USA300, EMRSA-15, Hungarian/Brazilian etc.), and isolated from infections or from carriers were studied for biofilm formation under static and dynamic conditions. Static biofilms in microtitre plates were quantified colorimetrically. Dynamic biofilms (Bioflux 200, Fluxion, USA) were studied by confocal laser-scanning and time-lapse microscopy, and the total volume occupied by live/dead bacteria quantified by Volocity 5.4.1 (Improvision, UK). Results MRSA harbouring SCCmec IV produced significantly more biomass under static conditions than SCCmec I-III (P = 0.003), and those harbouring SCCmec II significantly less than those harbouring SCCmec I or III (P
ISSN:1932-6203
DOI:10.1371/journal.pone.0104561