Resident CD4+ T cells accumulate in lymphoid organs after prolonged antigen exposure

Effector and memory CD4 + T cells acquire distinct migratory properties depending on the type and location of the immune responses. Due to the highly dynamic nature of T cell circulation, the comprehensive analysis of these migratory routes requires dedicated experimental approaches. Here, we analys...

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Bibliographic Details
Published in:Nature communications 2014-09, Vol.5 (1), p.4821-4821, Article 4821
Main Authors: Ugur, Milas, Schulz, Olga, Menon, Manoj B., Krueger, Andreas, Pabst, Oliver
Format: Article
Language:English
Subjects:
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631/250/347
631/80/84
64/60
Animals
Antigens - immunology
CD4-Positive T-Lymphocytes - immunology
Cell Movement - immunology
Cell Tracking
DNA Primers - genetics
Flow Cytometry
Histological Techniques
Humanities and Social Sciences
Humans
Lymph Nodes - cytology
Mice
Mice, Inbred C57BL
multidisciplinary
Peyer's Patches - cytology
Real-Time Polymerase Chain Reaction
Science
Science (multidisciplinary)
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Summary:Effector and memory CD4 + T cells acquire distinct migratory properties depending on the type and location of the immune responses. Due to the highly dynamic nature of T cell circulation, the comprehensive analysis of these migratory routes requires dedicated experimental approaches. Here, we analyse the migration of effector/memory CD4 + T cells by long-term in vivo cell tracking. We identify a resident population of antigen-experienced CD4 + T cells that resides in lymph nodes and Peyer’s patches without circulation or proliferation. Resident CD4 + T cells constitute up to 50% of all effector/memory cells, including, but not limited to, follicular helper T cells. Furthermore, these non-circulating T cells possess a distinct T cell receptor repertoire and accumulate in Peyer’s patches after continuous oral antigen exposure. Our results provide the first direct evidence for a resident population of effector/memory CD4 + T cells that is retained in lymphoid tissues. T follicular helper cells are lymph node residents, but whether other CD4 + T cells also reside in lymph nodes is not clear. Here, the authors use photoconversion to examine T cell migration in lymph nodes and Peyer’s patches and identify a population of antigen-experienced CD4 + T cells retained in lymphoid tissue.
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms5821