Abundance and length polymorphism of microsatellite repeats in Beta vulgaris L

Simple sequence repeats (SSRs) are known to exhibit high degrees of variability even among closely related individuals. Their usage as nuclear genetic markers requires their conversion into sequence-tagged sites (STSs). In this paper we present the development of simple sequences as STSs for Beta vu...

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Bibliographic Details
Published in:Theoretical and applied genetics 1996-03, Vol.92 (3-4), p.326-333
Main Authors: Moerchen, M, Cuguen, J, Michaelis, G, Haenni, C, Saumitou-Laprade, P. (Lille Univ., Villeneuve d'Ascq (France). Lab. de Genetique et Evolution des Populations Vegetales)
Format: Article
Language:English
Subjects:
ADN
BETA VULGARIS
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA
Fundamental and applied biological sciences. Psychology
GENETIC POLYMORPHISM
Genetics of eukaryotes. Biological and molecular evolution
NUCLEOTIDE SEQUENCE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Vegetals
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Summary:Simple sequence repeats (SSRs) are known to exhibit high degrees of variability even among closely related individuals. Their usage as nuclear genetic markers requires their conversion into sequence-tagged sites (STSs). In this paper we present the development of simple sequences as STSs for Beta vulgaris. This species comprises wild, cultivated, and weedy forms; the latter are thought to originate from accidental hybridisation between the other two. Two partial genomic libraries were screened with simple sequence motifs (AT, CA, CT, ATT, GTG, and CA, CT, respectively). Clones of 22 CA, nine CT, eight ATT, and one GTG sequence were obtained. AT micro satellites were present in compound motifs, not recognised by the probe. Sequence comparisons revealed that 20 CA clones containing short motifs (800 bp). Genetic variability was high among wild beets, lower among cultivated beets, and intermediate among weed beets. One allele of each locus was found at high frequencies in cultivated beets and, to a lower extent, in weed beets. The combination of three polymorphic loci allowed the individual identification of 17/17 wild and 15/15 weed beets, and 21/32, mostly homozygous, cultivated beets.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00223675