The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes

The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard an...

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Bibliographic Details
Published in:Euphytica 1996, Vol.89 (2), p.229-234
Main Authors: Samec, P. (Academy of Sciences of the Czech Republic, Ceske Budejovice (CZECH REPUBLIC). Inst. of Plant Molecular Biology), Nasinec, V
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cultivars: description, identification, tests, catalogs
FILOGENIA
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
GENOTIPOS
GENOTYPE
GENOTYPES
IDENTIFICACION
IDENTIFICATION
PHYLOGENIE
PHYLOGENY
PISUM SATIVUM
TECHNOLOGIE
TECHNOLOGY
TECNOLOGIA
VARIEDADES
Varietal selection. Specialized plant breeding, plant breeding aims
VARIETE
VARIETIES
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Summary:The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution.
ISSN:0014-2336
1573-5060
DOI:10.1007/bf00034610