Monochloramine suppresses the proliferation of colorectal cancer cell line Caco-2 by both apoptosis and G2/M cell cycle arrest

The aim of this study was to assess a possible role of monochloramine (NH2Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco‐2. At concentrations ranging from 10 to 200 μM, NH2Cl (14–61% inhibition), but not hypochlorous...

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Bibliographic Details
Published in:Cell biochemistry and function 2014-03, Vol.32 (2), p.188-193
Main Authors: Kohda, Tetsuya, Sakuma, Satoru, Abe, Muneyuki, Fujimoto, Yohko
Format: Article
Language:English
Subjects:
Apoptosis - drug effects
Caco-2 Cells
Cell Proliferation - drug effects
Chloramines - metabolism
Chloramines - pharmacology
colon cancer
Colorectal Neoplasms - pathology
G2 Phase Cell Cycle Checkpoints - drug effects
Humans
monochloramine
oxidative stress
proliferation
reactive chlorine species
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Summary:The aim of this study was to assess a possible role of monochloramine (NH2Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco‐2. At concentrations ranging from 10 to 200 μM, NH2Cl (14–61% inhibition), but not hypochlorous acid, dose‐dependently inhibited the cell viability of Caco‐2 cells. Experiments utilizing methionine (a scavenger of NH2Cl), taurine‐chloramine and glutamine‐chloramine revealed that only NH2Cl affects the cancer cell proliferation among reactive chlorine species, with a relative specificity. Furthermore, flow‐cytometry experiments showed that the anti‐proliferative effect of NH2Cl is partially attributable to both apoptosis and G2/M cell cycle arrest. These results suggest that NH2Cl has the potential to suppress colorectal cancer cell proliferation. Copyright © 2013 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.2992