Subchronic Toxicity of Ingested 1,3-Dichloropropene in Rats and Mice

• Published in: Fundamental and applied toxicology 1996-08, Vol.32 (2), p.224-232
• Main Authors: Haut, K.T., Stebbins, K.E., Johnson, K.A., Shabrang, S.N., Stott, W.T.
• Format: Article
• Language: English
• Subjects: Administration, Oral; Allyl Compounds - administration & dosage; Allyl Compounds - toxicity; Animals; Biological and medical sciences; Body Weight; COMPOSICION DE LA SANGRE; COMPOSITION DU SANG; DIETA; ENFERMEDADES DE LA PIEL; ESTOMAC; ESTOMAGO; Feeding Behavior - drug effects; Female; FUMIGANT; FUMIGANTES; HISTOPATHOLOGIE; HISTOPATOLOGIA; Hydrocarbons, Chlorinated; Hyperplasia; INGESTION DE PIENSOS; Insecticides - toxicity; MALADIE DE LA PEAU; Male; Medical sciences; MEMBRANA MUCOSA; METHODE D'APPLICATION; METODOS DE APLICACION; Mice; MUQUEUSE; NEOPLASMAS; NEOPLASME; Organ Size; PARTES DEL CUERPO; PARTIE DU CORPS; PESO; Pesticides, fertilizers and other agrochemicals toxicology; POIDS; PRISE ALIMENTAIRE (ANIMAUX); RAT; RATA; RATON; Rats; Rats, Inbred F344; REGIME ALIMENTAIRE; SERUM SANGUIN; SOURIS; SUERO SANGUINEO; TOXICIDAD; TOXICITE; Toxicology
• ISSN: 0272-0590; 1095-6832
• DOI: 10.1006/faat.1996.0125

Male and female Fischer 344 rats and B6C3F1 mice (10/sex/dose group) were given 0, 5, 15, 50, or 100 mg/kg/day (rats) or 0, 15, 50, 100, or 175 (mice) mg/kg/day racemic 1,3-dichloropropene (1,3-D), respectively, via their diets for 13 weeks. Satellite groups of rats (recovery = 10 rats/sex/group) ingesting 0 or 100 mg/kg/day 1,3-D were provided control feed for an additional 4 weeks to examine recovery. The test material was stabilized in the feed by microencapsulation in a starch/sucrose matrix (80/20). The body weights of male and female rats ingesting ≥5 and ≥15 mg/kg/day, respectively, and of all treatment groups of mice were decreased relative to controls. The terminal body weights of high dose group rats and mice were decreased approximately 13–16%. A number of changes in serum biochemical parameters and decreases in organ weights accompanied the depressed body weights of these animals. Histologically, the only treatment-related change observed was a slight degree of basal cell hyperplasia and hyperkeratosis in the nonglandular portion of the stomachs of a majority of male and female rats ingesting ≥15 mg/kg/day. After the 4-week recovery period, most treatment-related changes were noted to be reversible in nature. No treatment-related histopathological changes were observed in the tissues of treated mice. Based upon relatively slight depressions in body weights at the lowest dosages tested, the no-observed-adverse-effect levels for male rats and both sexes of mice were determined to be 5 mg/kg/day and 15 mg/kg/day, respectively. A no-observed-effect level of 5 mg/kg/day was established for female rats.