Isolation and characterization of novel salmon microsatellite loci: cross-species amplification and population genetic applications

Twenty-two variable number of tandem repeat microsatellite dinucleotide repeat loci were cloned from sockeye salmon (Oncorhynchus nerka) partial genomic libraries. Characteristics and optimal polymerase chain reaction (PCR) conditions were defined for each locus. The degree of conservation of sequen...

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Bibliographic Details
Published in:Canadian journal of fisheries and aquatic sciences 1996-04, Vol.53 (4), p.833-841
Main Authors: Scribner, K T, Gust, J R, Fields, R L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Freshwater
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Marine
Oncorhynchus nerka
Oncorhynchus tshawytscha
Population genetics, reproduction patterns
Vertebrata
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Summary:Twenty-two variable number of tandem repeat microsatellite dinucleotide repeat loci were cloned from sockeye salmon (Oncorhynchus nerka) partial genomic libraries. Characteristics and optimal polymerase chain reaction (PCR) conditions were defined for each locus. The degree of conservation of sequences flanking microsatellite repeat motifs and the utility of heterologous PCR primers for analyses in closely related taxa was tested using 10 salmonid species from four genera. Nearly all microsatellite primers produce amplification products in multiple species, suggesting broad application in salmonid research. The utility of these loci for population genetic studies was tested using individuals from 3 spawning populations of chinook salmon (O. tshawytscha) from the Yukon River, Yukon Territories. Twelve of 16 loci screened were polymorphic. Genetic distance estimates between populations were concordant with results from a previous allozyme survey of these same populations.
ISSN:0706-652X
1205-7533
DOI:10.1139/f95-254