Isolation and identification of cobalt- and caesium-resistant bacteria from a nuclear fuel storage pond

Abstract One of the issues facing the nuclear power industry is how to store spent nuclear fuel which is contaminated with radionuclides produced during nuclear fission, including caesium (134Cs+, 135Cs+ and 137Cs+) and cobalt (60Co2+). In this study, we have isolated Co2+- and Cs+-resistant bacteri...

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Bibliographic Details
Published in:FEMS microbiology letters 2014-10, Vol.359 (1), p.81-84
Main Authors: Dekker, Linda, Osborne, Thomas H., Santini, Joanne M.
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - drug effects
Bacteria - isolation & purification
Bioremediation
caesium
Cesium - metabolism
Cesium radioisotopes
Cluster Analysis
Cobalt
Cobalt - metabolism
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Drug Resistance, Bacterial
Microbiology
Molecular Sequence Data
Nuclear energy
Phylogeny
Ponds
Ponds - microbiology
Radioactive Waste
radionuclide
resistance
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:Abstract One of the issues facing the nuclear power industry is how to store spent nuclear fuel which is contaminated with radionuclides produced during nuclear fission, including caesium (134Cs+, 135Cs+ and 137Cs+) and cobalt (60Co2+). In this study, we have isolated Co2+- and Cs+-resistant bacteria from water collected from a nuclear fuel storage pond. The most resistant Cs+ and Co2+ isolates grew in the presence of 500 mM CsCl and 3 mM CoCl2. Strain Cs67-2 is resistant to fourfold more Cs+ than Cupriavidus metallidurans str. CH34 making it the most Cs+-resistant strain identified to date. The Cs+-resistant isolates were closely related to bacteria in the Serratia and Yersinia genera, while the Co2+-resistant isolates were closely related to the Curvibacter and Tardiphaga genera. These new isolates could be used for bioremediation. This study has identified bacteria from a nuclear fuel storage pond that are resistant to high concentrations of Co2+ or Cs+ and could be useful for nuclear fuel remediation. Graphical Abstract Figure. This study has identified bacteria from a nuclear fuel storage pond that are resistant to high concentrations of Co2+ or Cs+ and could be useful for nuclear fuel remediation.
ISSN:0378-1097
1574-6968
DOI:10.1111/1574-6968.12562