The Rhizobium strain-dependent efficiency of the plant infection dilution frequency technique, as encountered for pigeon pea

Soil samples were inoculated with different levels (10¹ to 10⁷ cells g⁻¹ soil) of A₇ and RM₇ (a streptomycin-resistant mutant of A₇) strains of pigeon pea Rhizobium. The populations of applied rhizobia in soil were immediately investigated with the plant infection dilution frequency technique (PIT)....

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Bibliographic Details
Published in:Plant and soil 1991-02, Vol.131 (1), p.147-149
Main Authors: Sidhu, B.S. (Punjab Agricultural Univ., Ludhiana (India). Dept. of Soils), Beri, V
Format: Article
Language:English
Subjects:
Agricultural soils
Agrology
Agronomy. Soil science and plant productions
antibiotic resistance
Biological and medical sciences
CAJANUS CAJAN
Economic plant physiology
ESTREPTOMICINA
Fundamental and applied biological sciences. Psychology
Infections
INOCULACION
INOCULATION
INOCULATION METHODS
Peas
Plants
resistant mutant
RHIZOBIUM
rhizosphere
Short communications
SOIL
Soil inoculation
Soil microorganisms
Soil samples
Soil treatment
SOL
STREPTOMYCIN
STREPTOMYCINE
SUELO
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Summary:Soil samples were inoculated with different levels (10¹ to 10⁷ cells g⁻¹ soil) of A₇ and RM₇ (a streptomycin-resistant mutant of A₇) strains of pigeon pea Rhizobium. The populations of applied rhizobia in soil were immediately investigated with the plant infection dilution frequency technique (PIT). The results showed that PIT failed to detect the population up to the level of 4.1 × 10³ cells of A₇ strain g⁻¹ soil, whereas it started to detect the RM₇ population when applied at 3.4 × 10³ cells g⁻¹ soil. Keeping the skips negative, the PIT detected 5.8 × 10¹ and 1.0 × 10³ cells out of the applied 4.1 × 10⁵ and 4.1 × 10⁷ cells of A₇ g⁻¹ soil, respectively, whereas 1.7 × 10¹, 1.0 × 10³ and 5.8 × 10⁴ cells of RM₇ were detectable out of the applied 3.4 × 10³, 3.4 × 10⁵ and 3.4 × 10⁷ cells g⁻¹ soil, respectively. These results indicate that the method counts the population of the RM₇ strain more efficiently than that of the A₇ strain when equal numbers of cells of these strains are added to the soil. This variation in efficiency was ascribed to the variable antagonistic effect of soil microorganisms on the Rhizobium.
ISSN:0032-079X
1573-5036
DOI:10.1007/BF00010430