Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species

Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of Gaeumannomyces graminis var. tritici, G. g. var. graminis, G. g. var. avenae, G. incrustans, and G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymor...

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Bibliographic Details
Published in:Soil biology & biochemistry 1996-06, Vol.28 (6), p.703-710
Main Authors: Fouly, Hanafy M., Wilkinson, Henry T., Domier, Leslie L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Gaeumannomyces
Generalities. Techniques
Phytopathology. Animal pests. Plant and forest protection
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Summary:Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of Gaeumannomyces graminis var. tritici, G. g. var. graminis, G. g. var. avenae, G. incrustans, and G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish G. graminis, G. incrustans, and G. cylindrosporous. Banding patterns were similar within isolates of G. g. var. tritici, G. g. var. avenae and G. incrustans. G. g. var. graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that G. g. var. tritici isolates were more closely related to G. g. var. avenae than to G. g. var. graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of Gaeumannomyces species and varieties.
ISSN:0038-0717
1879-3428
DOI:10.1016/0038-0717(95)00167-0