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Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species
Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of Gaeumannomyces graminis var. tritici, G. g. var. graminis, G. g. var. avenae, G. incrustans, and G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymor...
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| Published in: | Soil biology & biochemistry 1996-06, Vol.28 (6), p.703-710 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c430t-1053130f6b223ab96e124051c93c1bda05df65f45fe6bf7de32ca3423c50c4fa3 |
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| container_start_page | 703 |
| container_title | Soil biology & biochemistry |
| container_volume | 28 |
| creator | Fouly, Hanafy M. Wilkinson, Henry T. Domier, Leslie L. |
| description | Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of
Gaeumannomyces graminis var.
tritici, G. g. var.
graminis, G. g. var.
avenae, G. incrustans, and
G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish
G. graminis, G. incrustans, and
G. cylindrosporous. Banding patterns were similar within isolates of
G. g. var.
tritici, G. g. var.
avenae and
G. incrustans. G. g. var.
graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that
G. g. var.
tritici isolates were more closely related to
G. g. var.
avenae than to
G. g. var.
graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of
Gaeumannomyces species and varieties. |
| doi_str_mv | 10.1016/0038-0717(95)00167-0 |
| format | article |
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Gaeumannomyces graminis var.
tritici, G. g. var.
graminis, G. g. var.
avenae, G. incrustans, and
G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish
G. graminis, G. incrustans, and
G. cylindrosporous. Banding patterns were similar within isolates of
G. g. var.
tritici, G. g. var.
avenae and
G. incrustans. G. g. var.
graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that
G. g. var.
tritici isolates were more closely related to
G. g. var.
avenae than to
G. g. var.
graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of
Gaeumannomyces species and varieties.</description><identifier>ISSN: 0038-0717</identifier><identifier>EISSN: 1879-3428</identifier><identifier>DOI: 10.1016/0038-0717(95)00167-0</identifier><identifier>CODEN: SBIOAH</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Gaeumannomyces ; Generalities. Techniques ; Phytopathology. Animal pests. Plant and forest protection</subject><ispartof>Soil biology & biochemistry, 1996-06, Vol.28 (6), p.703-710</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-1053130f6b223ab96e124051c93c1bda05df65f45fe6bf7de32ca3423c50c4fa3</citedby><cites>FETCH-LOGICAL-c430t-1053130f6b223ab96e124051c93c1bda05df65f45fe6bf7de32ca3423c50c4fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3107414$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Fouly, Hanafy M.</creatorcontrib><creatorcontrib>Wilkinson, Henry T.</creatorcontrib><creatorcontrib>Domier, Leslie L.</creatorcontrib><title>Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species</title><title>Soil biology & biochemistry</title><description>Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of
Gaeumannomyces graminis var.
tritici, G. g. var.
graminis, G. g. var.
avenae, G. incrustans, and
G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish
G. graminis, G. incrustans, and
G. cylindrosporous. Banding patterns were similar within isolates of
G. g. var.
tritici, G. g. var.
avenae and
G. incrustans. G. g. var.
graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that
G. g. var.
tritici isolates were more closely related to
G. g. var.
avenae than to
G. g. var.
graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of
Gaeumannomyces species and varieties.</description><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Gaeumannomyces</subject><subject>Generalities. Techniques</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><issn>0038-0717</issn><issn>1879-3428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kMFqGzEQhkVJoU7SN-hBh1DiwzYzK2nXeykYp3UKoQmlOfUgZO2IKuyuNtI64LePHAcfcxoYvv8f5mPsC8I3BKyuAMSigBrry0bNIW_qAj6wGS7qphCyXJyw2RH5xE5TegSAUqGYsX8PiXhwPJqhDT03_dh556nlY-h2fYjjf2_59e8lv_yzvL-ecxci9y0NU6asmXwY9um1oW1vhiH0O0uJp5Gsp3TOPjrTJfr8Ns_Yw88ff1c3xe3d-tdqeVtYKWAqEJRAAa7alKUwm6YiLCUotI2wuGkNqNZVyknlqNq4uiVRWpPfElaBlc6IM_b10DvG8LSlNOneJ0tdZwYK26RRLRqJqDIoD6CNIaVITo_R9ybuNILem9R7TXqvSTdKv5rUkGMXb_0mWdO57Mr6dMwKhFqizNj3A0b512dPUadsYbDU-kh20m3w7995AXhihno</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Fouly, Hanafy M.</creator><creator>Wilkinson, Henry T.</creator><creator>Domier, Leslie L.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19960601</creationdate><title>Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species</title><author>Fouly, Hanafy M. ; Wilkinson, Henry T. ; Domier, Leslie L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-1053130f6b223ab96e124051c93c1bda05df65f45fe6bf7de32ca3423c50c4fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Gaeumannomyces</topic><topic>Generalities. Techniques</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fouly, Hanafy M.</creatorcontrib><creatorcontrib>Wilkinson, Henry T.</creatorcontrib><creatorcontrib>Domier, Leslie L.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Soil biology & biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fouly, Hanafy M.</au><au>Wilkinson, Henry T.</au><au>Domier, Leslie L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species</atitle><jtitle>Soil biology & biochemistry</jtitle><date>1996-06-01</date><risdate>1996</risdate><volume>28</volume><issue>6</issue><spage>703</spage><epage>710</epage><pages>703-710</pages><issn>0038-0717</issn><eissn>1879-3428</eissn><coden>SBIOAH</coden><abstract>Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of
Gaeumannomyces graminis var.
tritici, G. g. var.
graminis, G. g. var.
avenae, G. incrustans, and
G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish
G. graminis, G. incrustans, and
G. cylindrosporous. Banding patterns were similar within isolates of
G. g. var.
tritici, G. g. var.
avenae and
G. incrustans. G. g. var.
graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that
G. g. var.
tritici isolates were more closely related to
G. g. var.
avenae than to
G. g. var.
graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of
Gaeumannomyces species and varieties.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><doi>10.1016/0038-0717(95)00167-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0038-0717 |
| ispartof | Soil biology & biochemistry, 1996-06, Vol.28 (6), p.703-710 |
| issn | 0038-0717 1879-3428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15894115 |
| source | ScienceDirect Journals |
| subjects | Biological and medical sciences Fundamental and applied biological sciences. Psychology Fungal plant pathogens Gaeumannomyces Generalities. Techniques Phytopathology. Animal pests. Plant and forest protection |
| title | Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species |
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