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Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA
We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (13), p.5744-5748 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c549t-8b8a9540a828c64564d35423f055e7b3ed36062a101dcff4ff6fc47ad0b843a33 |
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| container_end_page | 5748 |
| container_issue | 13 |
| container_start_page | 5744 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 88 |
| creator | Campbell, Colin Gulati, Rajiv Nandi, Asit K. Floy, Kimberly Hieter, Philip Kucherlapati, Raju S. |
| description | We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships. |
| doi_str_mv | 10.1073/pnas.88.13.5744 |
| format | article |
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A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.13.5744</identifier><identifier>PMID: 2062854</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550400 - Genetics ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; CHROMOSOMAL ABERRATIONS ; Chromosome Deletion ; CHROMOSOMES ; Cloning, Molecular - methods ; Crossovers ; deletion ; DNA ; DNA probes ; ELECTROPHORESIS ; Enzymes ; EUMYCOTA ; Exons ; Fundamental and applied biological sciences. Psychology ; FUNGI ; GENE RECOMBINATION ; Genes ; Genetic Vectors ; Genic rearrangement. Recombination. Transposable element ; HETEROCHROMOSOMES ; HUMAN X CHROMOSOME ; Humans ; In Vitro Techniques ; MAMMALS ; MAN ; Mathematical vectors ; MICROORGANISMS ; Molecular and cellular biology ; Molecular genetics ; MUTATIONS ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; PLANTS ; Plasmids ; PRIMATES ; Recombination, Genetic ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping ; RFLPS ; SACCHAROMYCES ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; VERTEBRATES ; X CHROMOSOME ; X Chromosome - ultrastructure ; YEASTS</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-07, Vol.88 (13), p.5744-5748</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-8b8a9540a828c64564d35423f055e7b3ed36062a101dcff4ff6fc47ad0b843a33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357255$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357255$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768,58213,58446</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4954069$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2062854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5933083$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Campbell, Colin</creatorcontrib><creatorcontrib>Gulati, Rajiv</creatorcontrib><creatorcontrib>Nandi, Asit K.</creatorcontrib><creatorcontrib>Floy, Kimberly</creatorcontrib><creatorcontrib>Hieter, Philip</creatorcontrib><creatorcontrib>Kucherlapati, Raju S.</creatorcontrib><title>Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.</description><subject>550400 - Genetics</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>CHROMOSOMAL ABERRATIONS</subject><subject>Chromosome Deletion</subject><subject>CHROMOSOMES</subject><subject>Cloning, Molecular - methods</subject><subject>Crossovers</subject><subject>deletion</subject><subject>DNA</subject><subject>DNA probes</subject><subject>ELECTROPHORESIS</subject><subject>Enzymes</subject><subject>EUMYCOTA</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGI</subject><subject>GENE RECOMBINATION</subject><subject>Genes</subject><subject>Genetic Vectors</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>HETEROCHROMOSOMES</subject><subject>HUMAN X CHROMOSOME</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Mathematical vectors</subject><subject>MICROORGANISMS</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>MUTATIONS</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>PLANTS</subject><subject>Plasmids</subject><subject>PRIMATES</subject><subject>Recombination, Genetic</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Restriction Mapping</subject><subject>RFLPS</subject><subject>SACCHAROMYCES</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>VERTEBRATES</subject><subject>X CHROMOSOME</subject><subject>X Chromosome - ultrastructure</subject><subject>YEASTS</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS0EKkvhzAWQhRCcsrVjO3EkLqsttJWqcgAOnCyvM-56ldiL7SD670m0YaEXOFny-2bemxmEnlOypKRmZ3uv01LKJWVLUXP-AC0oaWhR8YY8RAtCyrqQvOSP0ZOUdoSQRkhygk5KUpVS8AXaXYCHqLMLHgeLNb6BlKHFnyE6SNPXlc8QU3bZ6Q6fQwcTm7Dz-BvolPEqZmedmdT1NoY-pNCPlWsd453zt_hy6LXH5zerp-iR1V2CZ_N7ir5-_PBlfVlcf7q4Wq-uCyN4kwu5kboRnGhZSlNxUfGWCV4yS4SAesOgZdWYXlNCW2Mtt7ayhte6JRvJmWbsFL0_9N0Pmx5aAz5H3al9dL2Odypop-4r3m3VbfihBB19x_LXh_IwDq2ScRnM1gTvwWQlGsaInDzezh4xfB_GlaneJQNdpz2EISlJKiHKpvkvSEVTEy7pCJ4dQBNDShHsMTAlajq1mk6tpFSUqenUY8XLv-c88vNtR_3NrOtkdGej9salI8anJVdTwlczNvX_rd7zefdPQNmh6zL8zCP54kDuUg7xTyAm6lII9gvVTdPg</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>Campbell, Colin</creator><creator>Gulati, Rajiv</creator><creator>Nandi, Asit K.</creator><creator>Floy, Kimberly</creator><creator>Hieter, Philip</creator><creator>Kucherlapati, Raju S.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19910701</creationdate><title>Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA</title><author>Campbell, Colin ; Gulati, Rajiv ; Nandi, Asit K. ; Floy, Kimberly ; Hieter, Philip ; Kucherlapati, Raju S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-8b8a9540a828c64564d35423f055e7b3ed36062a101dcff4ff6fc47ad0b843a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>550400 - Genetics</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>CHROMOSOMAL ABERRATIONS</topic><topic>Chromosome Deletion</topic><topic>CHROMOSOMES</topic><topic>Cloning, Molecular - methods</topic><topic>Crossovers</topic><topic>deletion</topic><topic>DNA</topic><topic>DNA probes</topic><topic>ELECTROPHORESIS</topic><topic>Enzymes</topic><topic>EUMYCOTA</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGI</topic><topic>GENE RECOMBINATION</topic><topic>Genes</topic><topic>Genetic Vectors</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>HETEROCHROMOSOMES</topic><topic>HUMAN X CHROMOSOME</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Mathematical vectors</topic><topic>MICROORGANISMS</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>MUTATIONS</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>PLANTS</topic><topic>Plasmids</topic><topic>PRIMATES</topic><topic>Recombination, Genetic</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Restriction Mapping</topic><topic>RFLPS</topic><topic>SACCHAROMYCES</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>VERTEBRATES</topic><topic>X CHROMOSOME</topic><topic>X Chromosome - ultrastructure</topic><topic>YEASTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campbell, Colin</creatorcontrib><creatorcontrib>Gulati, Rajiv</creatorcontrib><creatorcontrib>Nandi, Asit K.</creatorcontrib><creatorcontrib>Floy, Kimberly</creatorcontrib><creatorcontrib>Hieter, Philip</creatorcontrib><creatorcontrib>Kucherlapati, Raju S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campbell, Colin</au><au>Gulati, Rajiv</au><au>Nandi, Asit K.</au><au>Floy, Kimberly</au><au>Hieter, Philip</au><au>Kucherlapati, Raju S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>88</volume><issue>13</issue><spage>5744</spage><epage>5748</epage><pages>5744-5748</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2062854</pmid><doi>10.1073/pnas.88.13.5744</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-07, Vol.88 (13), p.5744-5748 |
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| subjects | 550400 - Genetics ANIMALS BASIC BIOLOGICAL SCIENCES Biological and medical sciences CHROMOSOMAL ABERRATIONS Chromosome Deletion CHROMOSOMES Cloning, Molecular - methods Crossovers deletion DNA DNA probes ELECTROPHORESIS Enzymes EUMYCOTA Exons Fundamental and applied biological sciences. Psychology FUNGI GENE RECOMBINATION Genes Genetic Vectors Genic rearrangement. Recombination. Transposable element HETEROCHROMOSOMES HUMAN X CHROMOSOME Humans In Vitro Techniques MAMMALS MAN Mathematical vectors MICROORGANISMS Molecular and cellular biology Molecular genetics MUTATIONS NUCLEIC ACIDS ORGANIC COMPOUNDS PLANTS Plasmids PRIMATES Recombination, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping RFLPS SACCHAROMYCES SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics VERTEBRATES X CHROMOSOME X Chromosome - ultrastructure YEASTS |
| title | Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA |
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