Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification

Improved recovery of vitrified currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) meristems and callus was obtained following 2 h pretreatment in sucrose, proline, abscisic acid-responsive proteins (RABP), or bovine serum albumin (BSA). Two hours immersion in 0.4 M RIB-SM prior to vitri...

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Bibliographic Details
Published in:Cryobiology 1997-05, Vol.34 (3), p.240-250
Main Authors: Luo, J. (Oregon State University, Corvallis, OR.), Reed, B.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
BIOLOGICAL PRESERVATION
Biotechnology
CONGELACION
CONGELATION
CONSERVACION BIOLOGICA
CONSERVATION BIOLOGIQUE
CRYOPRESERVATION
Eukaryotic cell cultures
FREEZING
Fundamental and applied biological sciences. Psychology
GERMOPLASMA
GERMPLASM
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Plant cells and fungal cells
RIBES
RIBES AUREUM
ribes ciliatum
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Summary:Improved recovery of vitrified currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) meristems and callus was obtained following 2 h pretreatment in sucrose, proline, abscisic acid-responsive proteins (RABP), or bovine serum albumin (BSA). Two hours immersion in 0.4 M RIB-SM prior to vitrification greatly improved the regrowth of meristems compared to 0, 1, 3, and 4 h immersion. Two hours immersion of meristems in 5 and 10% proline dissolved in 0.4 M RIB-SM significantly improved regrowth following vitrification. Initial tests with extracts of crude RABP from wheat seeds showed that regrowth of vitrified Ribes apical meristems improved after 2 h immersion pretreatment with the highest survival at 1% RABP. RABP preparations containing equivalent proteins (1% crude or 0.2% dialyzed RABP) had similar effects on regrowth, indicating that the effect was from the proteins rather than sugars and other carbohydrates in the crude RABP extracts. Pretreatments of meristems and callus with 5 or 10% proline, 1% crude RABP, or 1% BSA in 0.4 M sucrose solutions produced similar results. Pretreated meristems resumed growth 3 days after warming and reached the maximum regrowth at 1 week, compared to 2 weeks for non-pretreatment controls. We suggest using a 1% BSA pretreatment as the most economical and available of the materials tested.
ISSN:0011-2240
1090-2392
DOI:10.1006/cryo.1997.2000