Proteolytic activity associated with the nuclear scaffold. The effect of self-digestion on lamins

The inner aspect of the nuclear envelope is supported by a peripheral framework called the nuclear scaffold, which consists of both structural and functional proteins. Its major structural components, lamins A-C, form a highly polymerized and insoluble fibrous matrix during interphase of the cell cy...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-09, Vol.264 (25), p.15059-15065
Main Authors: TOKES, Z. A, CLAWSON, G. A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Caseins
Cell Nucleus - enzymology
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Hydrolysis
Interactions. Associations
Intermolecular phenomena
Lamins
Liver - drug effects
Liver - enzymology
Male
Molecular biophysics
Molecular Weight
Nuclear Proteins - isolation & purification
Nuclear Proteins - metabolism
Nucleoside-Triphosphatase
Peptide Hydrolases - isolation & purification
Peptide Hydrolases - metabolism
Phosphoric Monoester Hydrolases - metabolism
Protease Inhibitors - isolation & purification
proteolysis
Rats
Rats, Inbred Strains
Thioacetamide - pharmacology
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Summary:The inner aspect of the nuclear envelope is supported by a peripheral framework called the nuclear scaffold, which consists of both structural and functional proteins. Its major structural components, lamins A-C, form a highly polymerized and insoluble fibrous matrix during interphase of the cell cycle. Functional constituents of the scaffold include the 46-kDa nucleoside triphosphatase which is thought to participate in nucleocytoplasmic transport of mRNA. This 46-kDa component shares an amino-terminal sequence with lamins A and C, indicating that proteolytic remodeling of the nuclear scaffold may contribute to the generation of nucleoside triphosphatase activity (Clawson, G. A., Lackey, A., and Tökés, Z. A. (1988) Exp. Cell Res. 176, 180-186). We report here that neutral protease activity intimately associated with the nuclear scaffold is also a functional constituent. This activity has a considerable selectivity for lamins as shown by self-digestion of scaffold preparations, and it may participate in the remodeling of the nuclear scaffold after treatment with carcinogens.
ISSN:0021-9258
1083-351X