Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species

• Published in: Gene 2013-10, Vol.529 (1), p.21-26
• Main Authors: Bhattacharyya, Paromik, Kumaria, Suman, Kumar, Shrawan, Tandon, Pramod
• Format: Article
• Language: English
• Subjects: Cluster Analysis; Codon, Initiator - genetics; Codon, Initiator - metabolism; Dendrobium - classification; Dendrobium - genetics; Dendrobium nobile; DNA Primers; Endangered Species; Gene Flow; genes; Genetic Markers; Genetic Variation; Genotype; India; loci; Northeast India; Orchidaceae; PIC; Polymorphism, Genetic; SCoT; start codon; variance
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2013.07.096

Genetic variability in the wild genotypes of Dendrobium nobile Lindl. collected from different parts of Northeast India, was analyzed using a Start Codon Targeted (SCoT) marker system. A total of sixty individuals comprising of six natural populations were investigated for the existing natural genetic diversity. One hundred and thirty two (132) amplicons were produced by SCoT marker generating 96.21% polymorphism. The PIC value of the SCoT marker system was 0.78 and the Rp values of the primers ranged between 4.43 and 7.50. The percentage of polymorphic loci (Pp) ranging from 25% to 56.82%, Nei's gene diversity (h) from 0.08 to 0.15 with mean Nei's gene diversity of 0.28, and Shannon's information index (I) values ranging from 0.13 to 0.24 with an average value of 0.43 were recorded. The gene flow value (0.37) and the diversity among populations (0.57) demonstrated higher genetic variation among the populations. Analysis of molecular variance (AMOVA) showed 43.37% of variation within the populations, whereas 56.63% variation was recorded among the populations. Cluster analysis also reveals high genetic variation among the genotypes. Present investigation suggests the effectiveness of SCoT marker system to estimate the genetic diversity of D. nobile and that it can be seen as a preliminary point for future research on the population and evolutionary genetics of this endangered orchid species of medicinal importance. •First study on genetic diversity of Dendrobium nobile in Northeast India•Sixty genotypes across six wild populations were used for the study•SCoT markers revealed low gene flow•SCoT marker system can efficiently be use for genetic variation in orchids species