Organ-specific genotoxicity of the potent rodent bladder carcinogens o-anisidine and p-cresidine

We used a modification of the alkaline single-cell gel electrophoresis (SCG) (Comet) assay to evaluate the in vivo genotoxicity of two potent rodent bladder carcinogens, o-anisidine and p-cresidine, in mouse liver, lung, kidney, brain, and bone marrow, and in the mucosa of stomach, colon, and bladde...

Full description

Saved in:
Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 1998, Vol.412 (2), p.155-160
Main Authors: Sasaki, Yu F, Nishidate, Emi, Su, Ying Quan, Matsusaka, Naonori, Tsuda, Shuji, Susa, Nobuyuki, Furukawa, Yoshinori, Ueno, Shunji
Format: Article
Language:English
Subjects:
Alkaline single cell gel electrophoresis (SCG) assay
Biological and medical sciences
Bladder
Chemical mutagenesis
Genotoxicity
Medical sciences
Mouse multiple organs
o-Anisidine
p-Cresidine
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We used a modification of the alkaline single-cell gel electrophoresis (SCG) (Comet) assay to evaluate the in vivo genotoxicity of two potent rodent bladder carcinogens, o-anisidine and p-cresidine, in mouse liver, lung, kidney, brain, and bone marrow, and in the mucosa of stomach, colon, and bladder. Male CD-1 mice (8 weeks old) were sacrificed 3 and 24 h after oral administration of o-anisidine at 690 mg/kg or p-cresidine at 595 mg/kg. Both chemicals were dissolved in olive oil. Both chemicals yielded statistically significant DNA damage in bladder mucosa 3 and 24 h after treatment. o-Anisidine yielded DNA damage in the colon at 3 h, but not at 24 h. No significant effects were observed in any other organs. Our results suggest the importance of the urinary bladder as a sentinel organ for evaluating chemical genotoxicity in rodents.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(97)00183-6