Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction

The polymerase chain reaction (PCR) can be used to detect polymorphisms in the length of amplified sequences between the annealing sites of two synthetic DNA primers. When the distance varies between two individuals then the banding pattern generated by the PCR reaction is essentially a genetic poly...

Full description

Saved in:
Bibliographic Details
Published in:Theoretical and applied genetics 1991-08, Vol.82 (2), p.209-216
Main Authors: Weining, S. (Adelaide Univ., Glens Osmond, SA (Australia). Waite Agricultural Research Inst.. Centre for Cereal Biotechnology), Langridge, P
Format: Article
Language:English
Subjects:
ADN
ALFA AMILASA
ALPHA AMYLASE
Biological and medical sciences
CARTE GENETIQUE
Classical genetics, quantitative genetics, hybrids
DNA
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
GENETIC MAPS
GENETIC POLYMORPHISM
Genetics of eukaryotes. Biological and molecular evolution
HORDEUM VULGARE
MAPAS GENETICOS
NUCLEOTIDE SEQUENCE
POLIMERIZACION
POLIMORFISMO GENETICO
POLYMERISATION
POLYMERIZATION
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
RESTRICTION ENZYMES
SECALE CEREALE
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
TRITICUM AESTIVUM
Vegetals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The polymerase chain reaction (PCR) can be used to detect polymorphisms in the length of amplified sequences between the annealing sites of two synthetic DNA primers. When the distance varies between two individuals then the banding pattern generated by the PCR reaction is essentially a genetic polymorphism and can be mapped in the same way as other genetic markers. This procedure has been used in a number of eukaryotes. Here we report the use of PCR to detect genetic polymorphisms in cereals. Known gene sequences can be used to design primers and detect polymorphic PCR products. This is demonstrated with primers to the α-amylase gene family. A second approach is to use semi-random primers to target diverse regions of the genome. For this purpose the consensus sequences at the intron-exon splice junctions were used. The targeting of the intronexon splice junctions in conjunction with primers of random and defined sequences, such as α-amylase, provides a source of extensive variation in PCR products. These polymorphisms can be mapped as standard genetic markers.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226215