Survey of plastid RNA abundance during tomato fruit ripening: the amounts of RNA from the ORF 2280 region increase in chromoplasts

A comprehensive survey of the levels of plastid RNAs at progressive stages of tomato fruit ripening was conducted by hybridizing total RNA with labeled Pst I fragments that cover almost the entire tomato plastid genome and with gene-specific probes. Two different cultivars of tomato (Lycopersicon es...

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Bibliographic Details
Published in:Plant molecular biology 1991-12, Vol.17 (6), p.1179-1188
Main Authors: Richards, C.M. (Pennsylvania State Univ., University Park, PA (USA). Dept. of Molecular and Cell Biology), Hinman, S.B, Boyer, C.D, Hardison, R.C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ARN
Base Sequence
Biological and medical sciences
Blotting, Northern
Carotenoids - biosynthesis
Chloroplasts - metabolism
Chromosome Mapping
DNA
EXPRESION GENICA
EXPRESSION DES GENES
FRUITS
FRUTAS
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
LYCOPERSICON ESCULENTUM
MADURAMIENTO
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
MURISSAGE
Nicotiana - genetics
Open Reading Frames
Plant Development
Plants - genetics
Plants - metabolism
Plants, Toxic
PLASTE
PLASTIDIOS
PLASTIDS
RIPENING
RNA
RNA - metabolism
Sequence Alignment
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Summary:A comprehensive survey of the levels of plastid RNAs at progressive stages of tomato fruit ripening was conducted by hybridizing total RNA with labeled Pst I fragments that cover almost the entire tomato plastid genome and with gene-specific probes. Two different cultivars of tomato (Lycopersicon esculentum Mill.) were examined, Traveler 76 and Count II. One of the tomato probes, P7, revealed a pronounced increase in the amount of an 8.3 kb RNA in ripe fruit. The homologous region of the tobacco plastid genome contains several genes for ribosomal proteins and a large unidentified open reading frame (2280 codons). Little change was observed in the levels of many transcripts during ripening. However, in some cases (e.g. psbA and psbC/D) the amount of RNA decreased during ripening of Count II but showed little or no change in Traveler 76. The contrast between Traveler 76 and Count II tomatoes shows that the level of plastid transcripts can vary substantially during fruit ripening with no obvious effect on the chloroplast to chromoplast transition. The large RNA from the P7 region may encode a protein that functions predominantly in chromoplasts.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00028734