In vitro culture of Ginkgo

• Published in: In vitro cellular & developmental biology. Plant 1997-04, Vol.33 (2), p.125-127
• Main Authors: Camper, N.D. (Clemson University, Clemson, SC.), Coker, P.S, Wedge, D.E, Keese, R.J
• Format: Article
• Language: English
• Subjects: 2,4-D; 6-BENZILADENINA; 6-BENZYLADENINE; Agronomy. Soil science and plant productions; ANA; Auxins; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Biotechnology; CAL; CALLO; CALLUS; Cell culture techniques; COTILEDONES; COTYLEDON; COTYLEDONS; CULTIVO DE EMBRIONES; CULTURE D'EMBRYON; CULTURE MEDIA; DITERPENOIDE; DITERPENOIDS; DITERPENOS; Economic plant physiology; EMBRIONES VEGETALES; EMBRYO CULTURE; EMBRYON VEGETAL; Embryos; EXPLANT; EXPLANTES; EXPLANTS; Fundamental and applied biological sciences. Psychology; GINKGO BILOBA; Ginkgolides; ginkolides; In vitro culture; IN VITRO REGENERATION; KINETIN; KINETINE; MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; NAA; PLANT EMBRYOS; Plant physiology and development; Plant roots; Plants; QUINETINA; RACINE; RAICES; REGENERACION IN VITRO; REGENERATION IN VITRO; REGENERATIVE ABILITY; ROOTS; STEMS; TALLO; TIGE; Tissue culture techniques; Tissue cultures, protoplasts
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-997-0009-7

Ginkgo biloba L. is an important landscape tree, is resistant to insect, fungi and other pests, and produces a number of chemicals that have pharmaceutical properties (termed ginkgolides). Studies were initiated to establish an in vitro culture protocol for Ginkgo. Explants (intact embryos, embryos with cotyledons removed, and cotyledon tissue) were removed from disinfested seeds and cultured on Murashige and Skoog minimal organics medium with various combinations of either 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA) and either kinetin or benzyladenine (BA). Cultures were incubated in the light and morphological development was recorded. Both embryo and cotyledon explants produced callus (cotyledon tissue produced the most callus). Ginkgolides A and B were detected in callus tissue extracts. Intact embryo cultures initiated on media with 2,4-D plus NAA for 5 wk produced shoots and roots when transferred to media with 4.5 µM 2,4-D alone for an additional 5 wk. Plants were transferred from the 2,4-D media to pots and maintained in the greenhouse.