Allele-specific polymerase chain reaction (ASPCR) to detect the K65R mutation in minor variants of HIV-1 subtype C in treated patients not receiving TDF

The WHO has recommended tenofovir disoproxil fumarate (TDF) as one of the preferred first-line antiretrovirals and it was introduced into the South African National Guidelines in 2010. However, exposure to TDF can result in the development of the K65R resistance mutation, more commonly in subtype C...

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Bibliographic Details
Published in:Antiviral therapy 2014-01, Vol.19, p.A94-A94
Main Authors: Maharaj, S, Paredes, R, Sunpath, H, Kuritzkes, D R, Marconi, V C, Gordon, M
Format: Article
Language:English
Subjects:
Human immunodeficiency virus 1
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Summary:The WHO has recommended tenofovir disoproxil fumarate (TDF) as one of the preferred first-line antiretrovirals and it was introduced into the South African National Guidelines in 2010. However, exposure to TDF can result in the development of the K65R resistance mutation, more commonly in subtype C viruses, due to the unique polymorphisms found at codon 64, 65 and 66. This study investigated the prevalence of K65R in the minor viral population, using allele-specific PCR (ASPCR), in patients that did not receive TDF. RNA was extracted from stored plasma samples from a subset of participants in the SA Resistance Cohort Study (SARCS) which ended enrollment in 2006, prior to the introduction of TDF. Minority K65R mutations could impact on future TDF containing ARV regimens. Therefore, more sensitive assays such as ASPCR should be considered in order to screen for this mutation before starting a TDF based regimen.
ISSN:1359-6535