Approach to distribution and accumulation of dibutyl phthalate in rats by immunoassay

• Published in: Food and chemical toxicology 2013-06, Vol.56, p.18-27
• Main Authors: Zeng, Qiang, Wei, Chenxi, Wu, Yang, Li, Ke, Ding, Shumao, Yuan, Junlin, Yang, Xu, Chen, Mingqing
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Bio-accumulation; Biological and medical sciences; Dibutyl phthalate; Dibutyl Phthalate - pharmacokinetics; Distribution; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; fluorescent antibody technique; Fluorescent Antibody Technique - methods; food intake; hair follicles; Immunofluorescence assay; Kidney - metabolism; kidneys; liver; Liver - metabolism; Male; Medical sciences; monoclonal antibodies; Rats; Rats, Wistar; Semi-volatile organic compounds; stomach; Stomach - metabolism; sweat glands; testes; Testis - metabolism; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2013.01.045

[Display omitted] ► A fast, simple and economical analytical method to detect DBP in vivo was developed. ► DBP was determined by two novel approaches with a MAb specific to DBP in rats. ► IMF provides with a visual approach to detect distribution and content of DBP. ► The accumulations of DBP through dermal exposure were less than that of oral route. ► Most of DBP was metabolized in 2 or 3days. Dibutyl phthalate (DBP) is mainly taken up by the general population from food intake. To estimate intake of phthalates, determining distribution and accumulation of DBP in biological materials was a critical need. In this work, we set up two novel approaches with a monoclonal antibody specific to DBP to determine the distribution and accumulation of DBP in vivo. The contents of DBP in liver, kidney, stomach and testes were detected by immunofluorescence assays and indirect competitive ELISA. This data give directly evidence that indicates the distribution and accumulation of DBP in vivo. Double-label immunofluorescence assay provides with a visual approach to determination of the distribution and accumulation of DBP. It indicated that DBP accumulated in subcutaneous tissue such as sweat gland, hair follicle. Both of immunofluorescence assay and ELISA can be used to detect the content of DBP in biological materials. Our assays showed that DBP accumulated in viscera being rich in fat, such as liver, kidney and could overcome physiological barriers to penetrate testes. The date suggested that the accumulations of DBP exposed through dermal route were less than that of oral route and most of DBP was metabolized in 2 or 3days.