Effect of physiological stress on expression of glucose transporter 2 in liver of the wood frog, Rana sylvatica

Glucose transporters (GLUTs) have been implicated in the survival of various physiological stresses in mammals; however, little is known about the role of these proteins in stress tolerance in lower vertebrates. The wood frog (Rana sylvatica), which survives multiple winter‐related stresses by copio...

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Published in:Journal of experimental zoology. Part A, Ecological and integrative physiology Ecological and integrative physiology, 2014-12, Vol.321 (10), p.566-576
Main Authors: Rosendale, Andrew J., Lee Jr, Richard E., Costanzo, Jon P.
Format: Article
Language:English
Subjects:
Animals
Dehydration
Freezing
Gene Expression Regulation
Glucose - metabolism
Glucose Transporter Type 2 - metabolism
Hypoxia - metabolism
Liver - metabolism
Rana sylvatica
Ranidae - metabolism
Ranidae - physiology
Real-Time Polymerase Chain Reaction
RNA, Messenger
Seasons
Stress, Physiological
Urea - metabolism
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Summary:Glucose transporters (GLUTs) have been implicated in the survival of various physiological stresses in mammals; however, little is known about the role of these proteins in stress tolerance in lower vertebrates. The wood frog (Rana sylvatica), which survives multiple winter‐related stresses by copiously mobilizing hepatic glycogen stores, is an interesting subject for the study of glucose transport in amphibians. We examined the effects of several physiological stresses on GLUT2 protein and mRNA levels in the liver of R. sylvatica. Using immunoblotting techniques to measure relative GLUT2 abundance, we found that GLUT2 numbers increased in response to organismal freezing, hypoxia exposure, and glucose loading; whereas, experimental dehydration and urea loading did not affect GLUT2 abundance. GLUT2 mRNA levels, assessed using quantitative real‐time polymerase chain reaction, changed in accordance with protein abundance for most stresses, indicating that transcriptional regulation of GLUT2 occurs in response to stress. Overall, hepatic GLUT2 seems to be important in stress survival in R. sylvatica and is regulated to meet the physiological need to accumulate glucose. J. Exp. Zool. 321A: 566–576, 2014. © 2014 Wiley Periodicals, Inc.
ISSN:1932-5223
2471-5638
1932-5231
2471-5646
DOI:10.1002/jez.1885