Diaporthe sclerotioides exhibits no host specificity among cucurbit species

Plant–fungal specificity between cucurbitaceous crops and Diaporthe sclerotioides, the causal agent of black root rot, was studied using cucumbers (Cucumis sativa), melons (Cucumis melo), pumpkins (Cucurbita maxima), watermelons (Citrullus lanatus) and bottlegourd (Lagenaria siceraria var. gourda)....

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Bibliographic Details
Published in:Plant pathology 2014-12, Vol.63 (6), p.1357-1364
Main Authors: Shishido, M, Ohashi, T, Momma, N
Format: Article
Language:English
Subjects:
Biological and medical sciences
Citrullus lanatus
crops
cucumbers
Cucumis melo
Cucumis sativus
Cucurbita maxima
Cucurbitaceae
Diaporthe
Diaporthe sclerotioides
DNA
Fundamental and applied biological sciences. Psychology
fungi
host specificity
hosts
hypocotyls
infectiveness
Lagenaria siceraria
pathogens
Phytopathology. Animal pests. Plant and forest protection
plant–microbe specificity
polymerase chain reaction
pumpkins
root rot
seedlings
virulence
watermelons
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Summary:Plant–fungal specificity between cucurbitaceous crops and Diaporthe sclerotioides, the causal agent of black root rot, was studied using cucumbers (Cucumis sativa), melons (Cucumis melo), pumpkins (Cucurbita maxima), watermelons (Citrullus lanatus) and bottlegourd (Lagenaria siceraria var. gourda). Twelve D. sclerotioides isolates from these cucurbit species were cross‐inoculated. The virulence of the isolates was evaluated as the area under the disease progress curve (AUDPC). All cucurbit species were susceptible to each isolate, but AUDPCs were significantly different among the hosts, with the order of greatest to least being melon, cucumber, watermelon, bottlegourd and finally, pumpkin. The infectiveness of isolates was assessed as the quantity of D. sclerotioides DNA detected in the hypocotyls of seedlings 2 weeks after inoculation using a real‐time PCR protocol. The fungal DNA quantities varied among the species in the same order as the AUDPCs. Whilst there were statistically significant correlations between the virulence and infectiveness of D. sclerotioides isolates in cucumbers, melons and bottlegourds, their coefficients of determination were not high (r² 
ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12201