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Efficacy of a food plant-based oral cholera toxin B subunit vaccine
Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G M1 -ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were res...
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| Published in: | Nature biotechnology 1998-03, Vol.16 (3), p.292-297 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| container_title | Nature biotechnology |
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| creator | Arakawa, T Chong, D.K.X Langridge, W.H.R |
| description | Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G
M1
-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G
M1
-ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin. |
| doi_str_mv | 10.1038/nbt0398-292 |
| format | article |
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M1
-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G
M1
-ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0398-292</identifier><identifier>PMID: 9528012</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Animals ; Antibodies - blood ; BACTERIAL TOXINS ; Bacteriology ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Cercopithecus aethiops ; Cholera - complications ; Cholera - immunology ; Cholera Toxin - genetics ; Cholera Toxin - immunology ; Diarrhea - complications ; Diarrhea - immunology ; Diarrhea - therapy ; Female ; Fundamental and applied biological sciences. Psychology ; G(M1) Ganglioside - metabolism ; Immune Sera ; Life Sciences ; Mice ; Mice, Inbred Strains ; Microbiology ; Mucous Membrane - immunology ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified - genetics ; research-article ; SOLANUM TUBEROSUM ; Solanum tuberosum - genetics ; TOXINAS BACTERIANAS ; TOXINE BACTERIENNE ; TRANSGENIC PLANTS ; VACCINE DEVELOPMENT ; Vaccines - administration & dosage ; Vaccines - genetics ; Vaccines - pharmacology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vero Cells - immunology ; Vero Cells - microbiology ; VIBRIO CHOLERAE</subject><ispartof>Nature biotechnology, 1998-03, Vol.16 (3), p.292-297</ispartof><rights>Nature Publishing Company 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-b4493fd67763a2180d40fcfe7bef2583f37fc681d6077be6c94f90f8c72c2e7c3</citedby><cites>FETCH-LOGICAL-c554t-b4493fd67763a2180d40fcfe7bef2583f37fc681d6077be6c94f90f8c72c2e7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2195033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9528012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arakawa, T</creatorcontrib><creatorcontrib>Chong, D.K.X</creatorcontrib><creatorcontrib>Langridge, W.H.R</creatorcontrib><title>Efficacy of a food plant-based oral cholera toxin B subunit vaccine</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G
M1
-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G
M1
-ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin.</description><subject>Agriculture</subject><subject>Animals</subject><subject>Antibodies - blood</subject><subject>BACTERIAL TOXINS</subject><subject>Bacteriology</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cercopithecus aethiops</subject><subject>Cholera - complications</subject><subject>Cholera - immunology</subject><subject>Cholera Toxin - genetics</subject><subject>Cholera Toxin - immunology</subject><subject>Diarrhea - complications</subject><subject>Diarrhea - immunology</subject><subject>Diarrhea - therapy</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G(M1) Ganglioside - metabolism</subject><subject>Immune Sera</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Microbiology</subject><subject>Mucous Membrane - immunology</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified - genetics</subject><subject>research-article</subject><subject>SOLANUM TUBEROSUM</subject><subject>Solanum tuberosum - genetics</subject><subject>TOXINAS BACTERIANAS</subject><subject>TOXINE BACTERIENNE</subject><subject>TRANSGENIC PLANTS</subject><subject>VACCINE DEVELOPMENT</subject><subject>Vaccines - administration & dosage</subject><subject>Vaccines - genetics</subject><subject>Vaccines - pharmacology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vero Cells - immunology</subject><subject>Vero Cells - microbiology</subject><subject>VIBRIO CHOLERAE</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNp9kc1LBCEYhyWK7fPUOTCILjXlx6jjsZbtA4IO1VkcR2tiVjedifrvM3bYutRJ8ffwe18eAdjH6AwjWp37ukdUVgWRZA1sYVbyAnPJ1_MdVaJAmPFNsJ3SK0KIl5xPwEQyUiFMtsB05lxrtPmEwUENXQgNXHTa90Wtk21giLqD5iV0NmrYh4_Ww0uYhnrwbQ_ftTGtt7tgw-ku2b3x3AFPV7PH6U1xd399O724KwxjZS4sS0ldw4XgVBNcoaZEzjgrausIq6ijwhle4YYjkd-4kaWTyFVGEEOsMHQHHC97FzG8DTb1at4mY7u8rg1DUphThpmUGTxZgiaGlKJ1ahHbuY6fCiP1rUyNylRWlumDsXao57ZZsaOjnB-NuU5Gdy5qb9q0wgiWDFGasdMllnLin21Ur2GIPgv5Y-rhEve6H6Jd1f36zJ_NnA5KP8c89ekBSykQJ1yKf3ImGMX0C4j0ne4</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Arakawa, T</creator><creator>Chong, D.K.X</creator><creator>Langridge, W.H.R</creator><general>Nature Publishing Group US</general><general>Nature</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>19980301</creationdate><title>Efficacy of a food plant-based oral cholera toxin B subunit vaccine</title><author>Arakawa, T ; Chong, D.K.X ; Langridge, W.H.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-b4493fd67763a2180d40fcfe7bef2583f37fc681d6077be6c94f90f8c72c2e7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agriculture</topic><topic>Animals</topic><topic>Antibodies - blood</topic><topic>BACTERIAL TOXINS</topic><topic>Bacteriology</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cercopithecus aethiops</topic><topic>Cholera - complications</topic><topic>Cholera - immunology</topic><topic>Cholera Toxin - genetics</topic><topic>Cholera Toxin - immunology</topic><topic>Diarrhea - complications</topic><topic>Diarrhea - immunology</topic><topic>Diarrhea - therapy</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G(M1) Ganglioside - metabolism</topic><topic>Immune Sera</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Microbiology</topic><topic>Mucous Membrane - immunology</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified - genetics</topic><topic>research-article</topic><topic>SOLANUM TUBEROSUM</topic><topic>Solanum tuberosum - genetics</topic><topic>TOXINAS BACTERIANAS</topic><topic>TOXINE BACTERIENNE</topic><topic>TRANSGENIC PLANTS</topic><topic>VACCINE DEVELOPMENT</topic><topic>Vaccines - administration & dosage</topic><topic>Vaccines - genetics</topic><topic>Vaccines - pharmacology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vero Cells - immunology</topic><topic>Vero Cells - microbiology</topic><topic>VIBRIO CHOLERAE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arakawa, T</creatorcontrib><creatorcontrib>Chong, D.K.X</creatorcontrib><creatorcontrib>Langridge, W.H.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arakawa, T</au><au>Chong, D.K.X</au><au>Langridge, W.H.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of a food plant-based oral cholera toxin B subunit vaccine</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>16</volume><issue>3</issue><spage>292</spage><epage>297</epage><pages>292-297</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G
M1
-ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G
M1
-ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>9528012</pmid><doi>10.1038/nbt0398-292</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-0156 |
| ispartof | Nature biotechnology, 1998-03, Vol.16 (3), p.292-297 |
| issn | 1087-0156 1546-1696 |
| language | eng |
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| source | Nature |
| subjects | Agriculture Animals Antibodies - blood BACTERIAL TOXINS Bacteriology Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cercopithecus aethiops Cholera - complications Cholera - immunology Cholera Toxin - genetics Cholera Toxin - immunology Diarrhea - complications Diarrhea - immunology Diarrhea - therapy Female Fundamental and applied biological sciences. Psychology G(M1) Ganglioside - metabolism Immune Sera Life Sciences Mice Mice, Inbred Strains Microbiology Mucous Membrane - immunology PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified - genetics research-article SOLANUM TUBEROSUM Solanum tuberosum - genetics TOXINAS BACTERIANAS TOXINE BACTERIENNE TRANSGENIC PLANTS VACCINE DEVELOPMENT Vaccines - administration & dosage Vaccines - genetics Vaccines - pharmacology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vero Cells - immunology Vero Cells - microbiology VIBRIO CHOLERAE |
| title | Efficacy of a food plant-based oral cholera toxin B subunit vaccine |
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